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A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes

Author

Listed:
  • Jianping Zhang

    (University of Sydney
    CSIRO Agriculture & Food)

  • Timothy C. Hewitt

    (University of Sydney
    CSIRO Agriculture & Food)

  • Willem H. P. Boshoff

    (University of the Free State)

  • Ian Dundas

    (University of Adelaide)

  • Narayana Upadhyaya

    (CSIRO Agriculture & Food)

  • Jianbo Li

    (University of Sydney)

  • Mehran Patpour

    (Aarhus University)

  • Sutha Chandramohan

    (CSIRO Agriculture & Food)

  • Zacharias A. Pretorius

    (University of the Free State)

  • Mogens Hovmøller

    (Aarhus University)

  • Wendelin Schnippenkoetter

    (CSIRO Agriculture & Food)

  • Robert F. Park

    (University of Sydney)

  • Rohit Mago

    (CSIRO Agriculture & Food)

  • Sambasivam Periyannan

    (CSIRO Agriculture & Food)

  • Dhara Bhatt

    (CSIRO Agriculture & Food)

  • Sami Hoxha

    (University of Sydney)

  • Soma Chakraborty

    (CSIRO Agriculture & Food)

  • Ming Luo

    (CSIRO Agriculture & Food)

  • Peter Dodds

    (CSIRO Agriculture & Food)

  • Burkhard Steuernagel

    (John Innes Centre, Norwich Research Park)

  • Brande B. H. Wulff

    (John Innes Centre, Norwich Research Park)

  • Michael Ayliffe

    (CSIRO Agriculture & Food)

  • Robert A. McIntosh

    (University of Sydney)

  • Peng Zhang

    (University of Sydney)

  • Evans S. Lagudah

    (University of Sydney
    CSIRO Agriculture & Food)

Abstract

The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 and Sr61, with both genes independently introduced as alien chromosome introgressions from tall wheat grass (Thinopyrum ponticum). Mutational genomics and targeted exome capture identify Sr26 and Sr61 as separate single genes that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. Sr61 orthologs are absent from current Thinopyrum elongatum and wheat pan genome sequences, contrasting with Sr26 where homologues are present. Using gene-specific markers, we validate the presence of both genes on a single recombinant alien segment developed in wheat. The co-location of these genes on a small non-recombinogenic segment simplifies their deployment as a gene stack and potentially enhances their resistance durability.

Suggested Citation

  • Jianping Zhang & Timothy C. Hewitt & Willem H. P. Boshoff & Ian Dundas & Narayana Upadhyaya & Jianbo Li & Mehran Patpour & Sutha Chandramohan & Zacharias A. Pretorius & Mogens Hovmøller & Wendelin Sch, 2021. "A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23738-0
    DOI: 10.1038/s41467-021-23738-0
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    Cited by:

    1. Guotai Yu & Oadi Matny & Nicolas Champouret & Burkhard Steuernagel & Matthew J. Moscou & Inmaculada Hernández-Pinzón & Phon Green & Sadiye Hayta & Mark Smedley & Wendy Harwood & Ngonidzashe Kangara & , 2022. "Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Fei Ni & Yanyan Zheng & Xiaoke Liu & Yang Yu & Guangqiang Zhang & Lynn Epstein & Xue Mao & Jingzheng Wu & Cuiling Yuan & Bo Lv & Haixia Yu & Jinlong Li & Qi Zhao & Qiyu Yang & Jiajun Liu & Juan Qi & D, 2023. "Sequencing trait-associated mutations to clone wheat rust-resistance gene YrNAM," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    3. Huagang He & Zhaozhao Chen & Renchun Fan & Jie Zhang & Shanying Zhu & Jiale Wang & Qianyuan Zhang & Anli Gao & Shuangjun Gong & Lu Zhang & Yanan Li & Yitong Zhao & Simon G. Krattinger & Qian-Hua Shen , 2024. "A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    4. Huanhuan Li & Wenqiang Men & Chao Ma & Qianwen Liu & Zhenjie Dong & Xiubin Tian & Chaoli Wang & Cheng Liu & Harsimardeep S. Gill & Pengtao Ma & Zhibin Zhang & Bao Liu & Yue Zhao & Sunish K. Sehgal & W, 2024. "Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    5. Jianping Zhang & Jayaveeramuthu Nirmala & Shisheng Chen & Matthias Jost & Burkhard Steuernagel & Mirka Karafiatova & Tim Hewitt & Hongna Li & Erena Edae & Keshav Sharma & Sami Hoxha & Dhara Bhatt & Re, 2023. "Single amino acid change alters specificity of the multi-allelic wheat stem rust resistance locus SR9," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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