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NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity

Author

Listed:
  • Timothy J. Hawkins

    (University of Durham)

  • Michaela Kopischke

    (Norwich Research Park
    LMU Munich Biocenter)

  • Patrick J. Duckney

    (University of Durham)

  • Katarzyna Rybak

    (LMU Munich Biocenter)

  • David A. Mentlak

    (University of Durham)

  • Johan T. M. Kroon

    (University of Durham)

  • Mai Thu Bui

    (Austrian Academy of Sciences, Vienna BioCenter)

  • A. Christine Richardson

    (University of Durham)

  • Mary Casey

    (Norwich Research Park)

  • Agnieszka Alexander

    (Norwich Research Park)

  • Geert Jaeger

    (VIB-University Ghent, Center for Plant System Biology)

  • Monika Kalde

    (Department of Plant Sciences, University of Oxford)

  • Ian Moore

    (Department of Plant Sciences, University of Oxford)

  • Yasin Dagdas

    (Austrian Academy of Sciences, Vienna BioCenter)

  • Patrick J. Hussey

    (University of Durham)

  • Silke Robatzek

    (Norwich Research Park
    LMU Munich Biocenter)

Abstract

Members of the NETWORKED (NET) family are involved in actin-membrane interactions. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal closure in plant immunity. NET4 proteins interact with F-actin and with members of the Rab7 GTPase RABG3 family through two distinct domains, allowing for simultaneous localization to actin filaments and the tonoplast. NET4 proteins interact with GTP-bound, active RABG3 members, suggesting their function being downstream effectors. We also show that RABG3b is critical for stomatal closure induced by microbial patterns. Taken together, we conclude that the actin cytoskeletal remodelling during stomatal closure involves a molecular link between actin filaments and the tonoplast, which is mediated by the NET4-RABG3b interaction. We propose that stomatal closure to microbial patterns involves the coordinated action of immune-triggered osmotic changes and actin cytoskeletal remodelling likely driving compact vacuolar morphologies.

Suggested Citation

  • Timothy J. Hawkins & Michaela Kopischke & Patrick J. Duckney & Katarzyna Rybak & David A. Mentlak & Johan T. M. Kroon & Mai Thu Bui & A. Christine Richardson & Mary Casey & Agnieszka Alexander & Geert, 2023. "NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41337-z
    DOI: 10.1038/s41467-023-41337-z
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    References listed on IDEAS

    as
    1. Minxia Zou & Mengmeng Guo & Zhaoyang Zhou & Bingxiao Wang & Qing Pan & Jiajing Li & Jian-Min Zhou & Jiejie Li, 2021. "MPK3- and MPK6-mediated VLN3 phosphorylation regulates actin dynamics during stomatal immunity in Arabidopsis," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Kathrin Thor & Shushu Jiang & Erwan Michard & Jeoffrey George & Sönke Scherzer & Shouguang Huang & Julian Dindas & Paul Derbyshire & Nuno Leitão & Thomas A. DeFalco & Philipp Köster & Kerri Hunter & S, 2020. "The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity," Nature, Nature, vol. 585(7826), pages 569-573, September.
    3. Yi-Ju Lu & Pai Li & Masaki Shimono & Alex Corrion & Takumi Higaki & Sheng Yang He & Brad Day, 2020. "Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    4. Marie Boudsocq & Matthew R. Willmann & Matthew McCormack & Horim Lee & Libo Shan & Ping He & Jenifer Bush & Shu-Hua Cheng & Jen Sheen, 2010. "Differential innate immune signalling via Ca2+ sensor protein kinases," Nature, Nature, vol. 464(7287), pages 418-422, March.
    5. Pengwei Wang & Roman Pleskot & Jingze Zang & Joanna Winkler & Jie Wang & Klaas Yperman & Tong Zhang & Kun Wang & Jinli Gong & Yajie Guan & Christine Richardson & Patrick Duckney & Michael Vandorpe & E, 2019. "Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
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    Cited by:

    1. Weinan Sun & Linjie Xia & Jinwu Deng & Simin Sun & Dandan Yue & Jiaqi You & Maojun Wang & Shuangxia Jin & Longfu Zhu & Keith Lindsey & Xianlong Zhang & Xiyan Yang, 2024. "Evolution and subfunctionalization of CIPK6 homologous genes in regulating cotton drought resistance," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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