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Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity

Author

Listed:
  • Yi-Ju Lu

    (Michigan State University)

  • Pai Li

    (Michigan State University
    Michigan State University)

  • Masaki Shimono

    (Michigan State University)

  • Alex Corrion

    (Michigan State University)

  • Takumi Higaki

    (Kumamoto University)

  • Sheng Yang He

    (Michigan State University
    Michigan State University
    Department of Energy Plant Research Laboratory
    Michigan State University)

  • Brad Day

    (Michigan State University)

Abstract

Pattern-triggered immunity and effector-triggered immunity are two primary forms of innate immunity in land plants. The molecular components and connecting nodes of pattern-triggered immunity and effector-triggered immunity are not fully understood. Here, we report that the Arabidopsis calcium-dependent protein kinase CPK3 is a key regulator of both pattern-triggered immunity and effector-triggered immunity. In vitro and in vivo phosphorylation assays, coupled with genetic and cell biology-based analyses, show that actin-depolymerization factor 4 (ADF4) is a physiological substrate of CPK3, and that phosphorylation of ADF4 by CPK3 governs actin cytoskeletal organization associated with pattern-triggered immunity. CPK3 regulates stomatal closure induced by flg22 and is required for resistance to Pst DC3000. Our data further demonstrates that CPK3 is required for resistance to Pst DC3000 carrying the effector AvrPphB. These results suggest that CPK3 is a missing link between cytoskeleton organization, pattern-triggered immunity and effector-triggered immunity.

Suggested Citation

  • Yi-Ju Lu & Pai Li & Masaki Shimono & Alex Corrion & Takumi Higaki & Sheng Yang He & Brad Day, 2020. "Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-20007-4
    DOI: 10.1038/s41467-020-20007-4
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    Cited by:

    1. Minxia Zou & Mengmeng Guo & Zhaoyang Zhou & Bingxiao Wang & Qing Pan & Jiajing Li & Jian-Min Zhou & Jiejie Li, 2021. "MPK3- and MPK6-mediated VLN3 phosphorylation regulates actin dynamics during stomatal immunity in Arabidopsis," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Timothy J. Hawkins & Michaela Kopischke & Patrick J. Duckney & Katarzyna Rybak & David A. Mentlak & Johan T. M. Kroon & Mai Thu Bui & A. Christine Richardson & Mary Casey & Agnieszka Alexander & Geert, 2023. "NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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