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Evolution and subfunctionalization of CIPK6 homologous genes in regulating cotton drought resistance

Author

Listed:
  • Weinan Sun

    (Huazhong Agricultural University)

  • Linjie Xia

    (Huazhong Agricultural University)

  • Jinwu Deng

    (Huazhong Agricultural University)

  • Simin Sun

    (Huazhong Agricultural University)

  • Dandan Yue

    (Huazhong Agricultural University)

  • Jiaqi You

    (Huazhong Agricultural University)

  • Maojun Wang

    (Huazhong Agricultural University
    Hubei Hongshan Laboratory)

  • Shuangxia Jin

    (Huazhong Agricultural University
    Hubei Hongshan Laboratory)

  • Longfu Zhu

    (Huazhong Agricultural University
    Hubei Hongshan Laboratory)

  • Keith Lindsey

    (Durham University)

  • Xianlong Zhang

    (Huazhong Agricultural University
    Hubei Hongshan Laboratory)

  • Xiyan Yang

    (Huazhong Agricultural University
    Hubei Hongshan Laboratory)

Abstract

The occurrence of whole-genome duplication or polyploidy may promote plant adaptability to harsh environments. Here, we clarify the evolutionary relationship of eight GhCIPK6 homologous genes in upland cotton (Gossypium hirsutum). Gene expression and interaction analyses indicate that GhCIPK6 homologous genes show significant functional changes after polyploidy. Among these, GhCIPK6D1 and GhCIPK6D3 are significantly up-regulated by drought stress. Functional studies reveal that high GhCIPK6D1 expression promotes cotton drought sensitivity, while GhCIPK6D3 expression promotes drought tolerance, indicating clear functional differentiation. Genetic and biochemical analyses confirm the synergistic negative and positive regulation of cotton drought resistance through GhCBL1A1-GhCIPK6D1 and GhCBL2A1-GhCIPK6D3, respectively, to regulate stomatal movement by controlling the directional flow of K+ in guard cells. These results reveal differentiated roles of GhCIPK6 homologous genes in response to drought stress in upland cotton following polyploidy. The work provides a different perspective for exploring the functionalization and subfunctionalization of duplicated genes in response to polyploidization.

Suggested Citation

  • Weinan Sun & Linjie Xia & Jinwu Deng & Simin Sun & Dandan Yue & Jiaqi You & Maojun Wang & Shuangxia Jin & Longfu Zhu & Keith Lindsey & Xianlong Zhang & Xiyan Yang, 2024. "Evolution and subfunctionalization of CIPK6 homologous genes in regulating cotton drought resistance," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50097-3
    DOI: 10.1038/s41467-024-50097-3
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    References listed on IDEAS

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    1. Timothy J. Hawkins & Michaela Kopischke & Patrick J. Duckney & Katarzyna Rybak & David A. Mentlak & Johan T. M. Kroon & Mai Thu Bui & A. Christine Richardson & Mary Casey & Agnieszka Alexander & Geert, 2023. "NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Kun-Lun Li & Ren-Jie Tang & Chao Wang & Sheng Luan, 2023. "Potassium nutrient status drives posttranslational regulation of a low-K response network in Arabidopsis," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. Shengyi Liu & Yumei Liu & Xinhua Yang & Chaobo Tong & David Edwards & Isobel A. P. Parkin & Meixia Zhao & Jianxin Ma & Jingyin Yu & Shunmou Huang & Xiyin Wang & Junyi Wang & Kun Lu & Zhiyuan Fang & Ia, 2014. "The Brassica oleracea genome reveals the asymmetrical evolution of polyploid genomes," Nature Communications, Nature, vol. 5(1), pages 1-11, September.
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