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The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity

Author

Listed:
  • Kathrin Thor

    (University of East Anglia, Norwich Research Park)

  • Shushu Jiang

    (University of East Anglia, Norwich Research Park
    Chinese Academy of Sciences)

  • Erwan Michard

    (University of Maryland, Department of Cell Biology and Molecular Genetics)

  • Jeoffrey George

    (University of East Anglia, Norwich Research Park
    University of Zurich)

  • Sönke Scherzer

    (University of Würzburg)

  • Shouguang Huang

    (University of Würzburg)

  • Julian Dindas

    (University of Zurich)

  • Paul Derbyshire

    (University of East Anglia, Norwich Research Park)

  • Nuno Leitão

    (John Innes Centre, Norwich Research Park
    Synthace Ltd)

  • Thomas A. DeFalco

    (University of East Anglia, Norwich Research Park
    University of Zurich)

  • Philipp Köster

    (University of Zurich)

  • Kerri Hunter

    (University of Helsinki)

  • Sachie Kimura

    (University of Helsinki
    Ritsumeikan University)

  • Julien Gronnier

    (University of East Anglia, Norwich Research Park
    University of Zurich)

  • Lena Stransfeld

    (University of East Anglia, Norwich Research Park
    University of Zurich)

  • Yasuhiro Kadota

    (University of East Anglia, Norwich Research Park
    Plant Immunity Research Group)

  • Christoph A. Bücherl

    (University of East Anglia, Norwich Research Park
    Dr. Friedrich Eberth Arzneimittel GmbH)

  • Myriam Charpentier

    (John Innes Centre, Norwich Research Park)

  • Michael Wrzaczek

    (University of Helsinki)

  • Daniel MacLean

    (University of East Anglia, Norwich Research Park)

  • Giles E. D. Oldroyd

    (John Innes Centre, Norwich Research Park
    Sainsbury Laboratory Cambridge University)

  • Frank L. H. Menke

    (University of East Anglia, Norwich Research Park)

  • M. Rob G. Roelfsema

    (University of Würzburg)

  • Rainer Hedrich

    (University of Würzburg)

  • José Feijó

    (University of Maryland, Department of Cell Biology and Molecular Genetics)

  • Cyril Zipfel

    (University of East Anglia, Norwich Research Park
    University of Zurich)

Abstract

Perception of biotic and abiotic stresses often leads to stomatal closure in plants1,2. Rapid influx of calcium ions (Ca2+) across the plasma membrane has an important role in this response, but the identity of the Ca2+ channels involved has remained elusive3,4. Here we report that the Arabidopsis thaliana Ca2+-permeable channel OSCA1.3 controls stomatal closure during immune signalling. OSCA1.3 is rapidly phosphorylated upon perception of pathogen-associated molecular patterns (PAMPs). Biochemical and quantitative phosphoproteomics analyses reveal that the immune receptor-associated cytosolic kinase BIK1 interacts with and phosphorylates the N-terminal cytosolic loop of OSCA1.3 within minutes of treatment with the peptidic PAMP flg22, which is derived from bacterial flagellin. Genetic and electrophysiological data reveal that OSCA1.3 is permeable to Ca2+, and that BIK1-mediated phosphorylation on its N terminus increases this channel activity. Notably, OSCA1.3 and its phosphorylation by BIK1 are critical for stomatal closure during immune signalling, and OSCA1.3 does not regulate stomatal closure upon perception of abscisic acid—a plant hormone associated with abiotic stresses. This study thus identifies a plant Ca2+ channel and its activation mechanisms underlying stomatal closure during immune signalling, and suggests specificity in Ca2+ influx mechanisms in response to different stresses.

Suggested Citation

  • Kathrin Thor & Shushu Jiang & Erwan Michard & Jeoffrey George & Sönke Scherzer & Shouguang Huang & Julian Dindas & Paul Derbyshire & Nuno Leitão & Thomas A. DeFalco & Philipp Köster & Kerri Hunter & S, 2020. "The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity," Nature, Nature, vol. 585(7826), pages 569-573, September.
  • Handle: RePEc:nat:nature:v:585:y:2020:i:7826:d:10.1038_s41586-020-2702-1
    DOI: 10.1038/s41586-020-2702-1
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    Citations

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    Cited by:

    1. Muhammad Awais Zahid & Nam Phuong Kieu & Frida Meijer Carlsen & Marit Lenman & Naga Charan Konakalla & Huanjie Yang & Sunmoon Jyakhwa & Jozef Mravec & Ramesh Vetukuri & Bent Larsen Petersen & Svante R, 2024. "Enhanced stress resilience in potato by deletion of Parakletos," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Youyou Lu & Xuan Zhang & Liyan Zhao & Hong Liu & Mi Yan & Xiaochen Zhang & Kenji Mochizuki & Shikuan Yang, 2023. "Metal-organic framework template-guided electrochemical lithography on substrates for SERS sensing applications," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    3. Jiaojiao Bai & Yuanyuan Zhou & Jianhang Sun & Kexin Chen & Yufang Han & Ranran Wang & Yanmin Zou & Mingshuo Du & Dongping Lu, 2023. "BIK1 protein homeostasis is maintained by the interplay of different ubiquitin ligases in immune signaling," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    4. Timothy J. Hawkins & Michaela Kopischke & Patrick J. Duckney & Katarzyna Rybak & David A. Mentlak & Johan T. M. Kroon & Mai Thu Bui & A. Christine Richardson & Mary Casey & Agnieszka Alexander & Geert, 2023. "NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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