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Phosphatidylinositol-4-phosphate controls autophagosome formation in Arabidopsis thaliana

Author

Listed:
  • Rodrigo Enrique Gomez

    (Univ. Bordeaux)

  • Clément Chambaud

    (Univ. Bordeaux)

  • Josselin Lupette

    (Univ. Bordeaux)

  • Julie Castets

    (Univ. Bordeaux)

  • Stéphanie Pascal

    (Univ. Bordeaux)

  • Lysiane Brocard

    (Univ. Bordeaux)

  • Lise Noack

    (Univ. Lyon, ENS de Lyon, CNRS, INRAE)

  • Yvon Jaillais

    (Univ. Lyon, ENS de Lyon, CNRS, INRAE)

  • Jérôme Joubès

    (Univ. Bordeaux)

  • Amélie Bernard

    (Univ. Bordeaux)

Abstract

Autophagy is an intracellular degradation mechanism critical for plant acclimation to environmental stresses. Central to autophagy is the formation of specialized vesicles, the autophagosomes, which target and deliver cargo to the lytic vacuole. How autophagosomes form in plant cells remains poorly understood. Here, we uncover the importance of the lipid phosphatidylinositol-4-phosphate in autophagy using pharmacological and genetical approaches. Combining biochemical and live-microscopy analyses, we show that PI4K activity is required for early stages of autophagosome formation. Further, our results show that the plasma membrane-localized PI4Kα1 is involved in autophagy and that a substantial portion of autophagy structures are found in proximity to the PI4P-enriched plasma membrane. Together, our study unravels critical insights into the molecular determinants of autophagy, proposing a model whereby the plasma membrane provides PI4P to support the proper assembly and expansion of the phagophore thus governing autophagosome formation in Arabidopsis.

Suggested Citation

  • Rodrigo Enrique Gomez & Clément Chambaud & Josselin Lupette & Julie Castets & Stéphanie Pascal & Lysiane Brocard & Lise Noack & Yvon Jaillais & Jérôme Joubès & Amélie Bernard, 2022. "Phosphatidylinositol-4-phosphate controls autophagosome formation in Arabidopsis thaliana," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32109-2
    DOI: 10.1038/s41467-022-32109-2
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    References listed on IDEAS

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    1. Yoko Ito & Nicolas Esnay & Matthieu Pierre Platre & Valérie Wattelet-Boyer & Lise C. Noack & Louise Fougère & Wilhelm Menzel & Stéphane Claverol & Laetitia Fouillen & Patrick Moreau & Yvon Jaillais & , 2021. "Sphingolipids mediate polar sorting of PIN2 through phosphoinositide consumption at the trans-Golgi network," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
    2. Pengwei Wang & Roman Pleskot & Jingze Zang & Joanna Winkler & Jie Wang & Klaas Yperman & Tong Zhang & Kun Wang & Jinli Gong & Yajie Guan & Christine Richardson & Patrick Duckney & Michael Vandorpe & E, 2019. "Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
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    Cited by:

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