IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-12782-6.html
   My bibliography  Save this article

Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery

Author

Listed:
  • Pengwei Wang

    (Durham University
    Huazhong Agricultural University)

  • Roman Pleskot

    (Ghent University
    VIB Center for Plant Systems Biology)

  • Jingze Zang

    (Durham University
    Huazhong Agricultural University)

  • Joanna Winkler

    (Ghent University
    VIB Center for Plant Systems Biology)

  • Jie Wang

    (Ghent University)

  • Klaas Yperman

    (Ghent University
    VIB Center for Plant Systems Biology)

  • Tong Zhang

    (Huazhong Agricultural University)

  • Kun Wang

    (Huazhong Agricultural University)

  • Jinli Gong

    (Huazhong Agricultural University)

  • Yajie Guan

    (Huazhong Agricultural University)

  • Christine Richardson

    (Durham University)

  • Patrick Duckney

    (Durham University)

  • Michael Vandorpe

    (Ghent University
    VIB Center for Plant Systems Biology)

  • Evelien Mylle

    (Ghent University
    VIB Center for Plant Systems Biology)

  • Jindriska Fiserova

    (Durham University
    Institute of Molecular Genetics CAS)

  • Daniel Van Damme

    (Ghent University
    VIB Center for Plant Systems Biology)

  • Patrick J. Hussey

    (Durham University)

Abstract

The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these proteins are also involved in actin cytoskeleton regulated autophagy. Both AtEH/Pan1 proteins localise to the plasma membrane and autophagosomes. Upon induction of autophagy, AtEH/Pan1 proteins recruit TPC and AP-2 subunits, clathrin, actin and ARP2/3 proteins to autophagosomes. Increased expression of AtEH/Pan1 proteins boosts autophagosome formation, suggesting independent and redundant pathways for actin-mediated autophagy in plants. Moreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either AtEH1/Pan1 or VAP27-1 makes plants more susceptible to nutrient depleted conditions, indicating that the autophagy pathway is perturbed. In conclusion, we identify the existence of an autophagy-dependent pathway in plants to degrade endocytic components, starting at the EPCS through the interaction among AtEH/Pan1, actin cytoskeleton and the EPCS resident protein VAP27-1.

Suggested Citation

  • Pengwei Wang & Roman Pleskot & Jingze Zang & Joanna Winkler & Jie Wang & Klaas Yperman & Tong Zhang & Kun Wang & Jinli Gong & Yajie Guan & Christine Richardson & Patrick Duckney & Michael Vandorpe & E, 2019. "Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12782-6
    DOI: 10.1038/s41467-019-12782-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-12782-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-12782-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Rodrigo Enrique Gomez & Clément Chambaud & Josselin Lupette & Julie Castets & Stéphanie Pascal & Lysiane Brocard & Lise Noack & Yvon Jaillais & Jérôme Joubès & Amélie Bernard, 2022. "Phosphatidylinositol-4-phosphate controls autophagosome formation in Arabidopsis thaliana," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Lin-lin Zhao & Ru Chen & Ziyu Bai & Junyi Liu & Yuhao Zhang & Yicheng Zhong & Meng-xiang Sun & Peng Zhao, 2024. "Autophagy-mediated degradation of integumentary tapetum is critical for embryo pattern formation," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    3. Chengyang Li & Patrick Duckney & Tong Zhang & Yanshu Fu & Xin Li & Johan Kroon & Geert Jaeger & Yunjiang Cheng & Patrick J. Hussey & Pengwei Wang, 2022. "TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    4. Timothy J. Hawkins & Michaela Kopischke & Patrick J. Duckney & Katarzyna Rybak & David A. Mentlak & Johan T. M. Kroon & Mai Thu Bui & A. Christine Richardson & Mary Casey & Agnieszka Alexander & Geert, 2023. "NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    5. José Cerca & Bent Petersen & José Miguel Lazaro-Guevara & Angel Rivera-Colón & Siri Birkeland & Joel Vizueta & Siyu Li & Qionghou Li & João Loureiro & Chatchai Kosawang & Patricia Jaramillo Díaz & Gon, 2022. "The genomic basis of the plant island syndrome in Darwin’s giant daisies," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    6. Tong Zhang & Yifan Li & Chengyang Li & Jingze Zang & Erlin Gao & Johan T. Kroon & Xiaolu Qu & Patrick J. Hussey & Pengwei Wang, 2023. "Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12782-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.