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Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley

Author

Listed:
  • Xiaohua Yao

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

  • Leping Su

    (Qinghai University, Xining, P.R. China
    Yan'an Institute of Agricultural Sciences, Yan'an, P.R. China)

  • Youhua Yao

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

  • Likun An

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

  • Yixiong Bai

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

  • Xin Li

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

  • Kunlun Wu

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

Abstract

2-hydroxyisoflavanone dehydratase (HID) plays an important role in isoflavone biosynthesis. In this study, HID was isolated from the seeds of the purple-grained Tibetan hulless barley variety Nerumuzha and the white-grained variety Kunlun 10. The HvnHID gene includes the 981 bp open reading frame and encodes a protein of 327 amino acids. It has a typical Abhydrolase_3 domain (78-306) and belongs to the carboxylesterase (CXE) family of the Abhydrolase_3 (α/β hydrolase) superfamily. There are eight nucleotide differences in the HvnHID coding sequence and two amino acid differences (one in the Abhydrolase_3 domain) between Nerumuzha and Kunlun 10. The HvnHID of hulless barley has the closest relationship with the HID in Hordeum vulgare, and the most distant relationship in Panicum hallii. At the early-mid stage of the seed colour development, the HvnHID expression levels in the purple and black seeds were significantly higher than in the white and blue ones (P < 0.01). During the seed colour development of purple-grained hulless barley, the expression of the key genes (HvnF3'H, HvnDRF, HvnANT1, and HvnGT) in the anthocyanidin biosynthetic pathway increased significantly, while the HvnHID expression decreased significantly (P < 0.01). Thus, it is likely that HvnHID negatively regulates the anthocyanidin biosynthesis. This result provides an important basis for further study of the biological functions of HvnHID in the anthocyanidin biosynthetic pathway.

Suggested Citation

  • Xiaohua Yao & Leping Su & Youhua Yao & Likun An & Yixiong Bai & Xin Li & Kunlun Wu, 2022. "Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 58(1), pages 1-9.
    • Handle: RePEc:caa:jnlcjg:v:58:y:2022:i:1:id:34-2021-cjgpb
    DOI: 10.17221/34/2021-CJGPB
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    1. Murukarthick Jayakodi & Sudharsan Padmarasu & Georg Haberer & Venkata Suresh Bonthala & Heidrun Gundlach & Cécile Monat & Thomas Lux & Nadia Kamal & Daniel Lang & Axel Himmelbach & Jennifer Ens & Xiao, 2020. "The barley pan-genome reveals the hidden legacy of mutation breeding," Nature, Nature, vol. 588(7837), pages 284-289, December.
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