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Continuous cellulosic bioethanol co-fermentation by immobilized Zymomonas mobilis and suspended Pichia stipitis in a two-stage process

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  • Wirawan, Ferdian
  • Cheng, Chieh-Lun
  • Lo, Yung-Chung
  • Chen, Chun-Yen
  • Chang, Jo-Shu
  • Leu, Shao-Yuan
  • Lee, Duu-Jong

Abstract

Bioethanol produced from lignocellulosic materials has been considered as one of the most promising fuels to replace fossil fuels. Immobilized yeasts or bacteria have been frequently used in continuous system due to its feasibility for repeated use with high biomass retention during the continuous process. In this study, continuous SHcF (separate hydrolysis and co-fermentation) and SScF (simultaneous saccharification and co-fermentation) were evaluated for ethanol production from alkaline pretreated sugarcane bagasse using Zymomonas mobilis (PVA immobilized cells) and Pichia stipitis (suspended cells). In SHcF fermentation, the ethanol yield and productivity of 0.36 g ethanol/g cellulose (corresponding to 70.65% of theoretical yield) and 1.868 g/L/h were achieved. In contrast, SScF system resulted in an ethanol yield of 0.414 g ethanol/g cellulose (corresponding to 81.17% of theoretical yield) and ethanol productivity of 0.705 g/L/h. The performance of the two systems are compared and discussed.

Suggested Citation

  • Wirawan, Ferdian & Cheng, Chieh-Lun & Lo, Yung-Chung & Chen, Chun-Yen & Chang, Jo-Shu & Leu, Shao-Yuan & Lee, Duu-Jong, 2020. "Continuous cellulosic bioethanol co-fermentation by immobilized Zymomonas mobilis and suspended Pichia stipitis in a two-stage process," Applied Energy, Elsevier, vol. 266(C).
    • Handle: RePEc:eee:appene:v:266:y:2020:i:c:s0306261920303834
    DOI: 10.1016/j.apenergy.2020.114871
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    1. Monte, J.R. & Brienzo, M. & Milagres, A.M.F., 2011. "Utilization of pineapple stem juice to enhance enzyme-hydrolytic efficiency for sugarcane bagasse after an optimized pre-treatment with alkaline peroxide," Applied Energy, Elsevier, vol. 88(1), pages 403-408, January.
    2. Pérez-Uresti, Salvador I. & Martín, Mariano & Jiménez-Gutiérrez, Arturo, 2019. "Estimation of renewable-based steam costs," Applied Energy, Elsevier, vol. 250(C), pages 1120-1131.
    3. Behera, Shuvashish & Mohanty, Rama Chandra & Ray, Ramesh Chandra, 2010. "Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae and Zymomonas mobilis," Applied Energy, Elsevier, vol. 87(7), pages 2352-2355, July.
    4. Wirawan, Ferdian & Cheng, Chieh-Lun & Kao, Wei-Chen & Lee, Duu-Jong & Chang, Jo-Shu, 2012. "Cellulosic ethanol production performance with SSF and SHF processes using immobilized Zymomonas mobilis," Applied Energy, Elsevier, vol. 100(C), pages 19-26.
    5. van Niekerk, A.S. & Drew, B. & Larsen, N. & Kay, P.J., 2019. "Influence of blends of diesel and renewable fuels on compression ignition engine emissions over transient engine conditions," Applied Energy, Elsevier, vol. 255(C).
    6. Zheng, Yi & Pan, Zhongli & Zhang, Ruihong & Wang, Donghai, 2009. "Enzymatic saccharification of dilute acid pretreated saline crops for fermentable sugar production," Applied Energy, Elsevier, vol. 86(11), pages 2459-2465, November.
    7. Cho, Seong-Heon & Kim, Juyeon & Han, Jeehoon & Lee, Daewon & Kim, Hyung Ju & Kim, Yong Tae & Cheng, Xun & Xu, Ye & Lee, Jechan & Kwon, Eilhann E., 2019. "Bioalcohol production from acidogenic products via a two-step process: A case study of butyric acid to butanol," Applied Energy, Elsevier, vol. 252(C), pages 1-1.
    8. Kesharwani, Rajkamal & Sun, Zeyi & Dagli, Cihan & Xiong, Haoyi, 2019. "Moving second generation biofuel manufacturing forward: Investigating economic viability and environmental sustainability considering two strategies for supply chain restructuring," Applied Energy, Elsevier, vol. 242(C), pages 1467-1496.
    9. Behera, Shuvashish & Kar, Shaktimay & Mohanty, Rama Chandra & Ray, Ramesh Chandra, 2010. "Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae cells immobilized in agar agar and Ca-alginate matrices," Applied Energy, Elsevier, vol. 87(1), pages 96-100, January.
    10. Wang, Pixiang & Chen, Yong Mei & Wang, Yifen & Lee, Yoon Y. & Zong, Wenming & Taylor, Steven & McDonald, Timothy & Wang, Yi, 2019. "Towards comprehensive lignocellulosic biomass utilization for bioenergy production: Efficient biobutanol production from acetic acid pretreated switchgrass with Clostridium saccharoperbutylacetonicum ," Applied Energy, Elsevier, vol. 236(C), pages 551-559.
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