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A fungal core effector exploits the OsPUX8B.2–OsCDC48-6 module to suppress plant immunity

Author

Listed:
  • Xuetao Shi

    (Chinese Academy of Agricultural Sciences
    Chinese Academy of Agricultural Sciences)

  • Xin Xie

    (Chinese Academy of Agricultural Sciences)

  • Yuanwen Guo

    (Chinese Academy of Agricultural Sciences)

  • Junqi Zhang

    (Chinese Academy of Agricultural Sciences)

  • Ziwen Gong

    (Chinese Academy of Agricultural Sciences
    Chinese Academy of Agricultural Sciences)

  • Kai Zhang

    (Chinese Academy of Agricultural Sciences)

  • Jie Mei

    (Chinese Academy of Agricultural Sciences
    Chinese Academy of Agricultural Sciences)

  • Xinyao Xia

    (Chinese Academy of Agricultural Sciences)

  • Haoxue Xia

    (Chinese Academy of Agricultural Sciences)

  • Na Ning

    (Chinese Academy of Agricultural Sciences)

  • Yutao Xiao

    (Chinese Academy of Agricultural Sciences)

  • Qing Yang

    (Chinese Academy of Agricultural Sciences)

  • Guo-Liang Wang

    (The Ohio State University)

  • Wende Liu

    (Chinese Academy of Agricultural Sciences)

Abstract

Proteins containing a ubiquitin regulatory X (UBX) domain are cofactors of Cell Division Cycle 48 (CDC48) and function in protein quality control. However, whether and how UBX-containing proteins participate in host–microbe interactions remain unclear. Here we show that MoNLE1, an effector from the fungal pathogen Magnaporthe oryzae, is a core virulence factor that suppresses rice immunity by specifically interfering with OsPUX8B.2. The UBX domain of OsPUX8B.2 is required for its binding to OsATG8 and OsCDC48-6 and controls its 26 S proteasome–dependent stability. OsPUX8B.2 and OsCDC48-6 positively regulate plant immunity against blast fungus, while the high-temperature tolerance heat-shock protein OsBHT, a putative cytoplasmic substrate of OsPUX8B.2–OsCDC48-6, negatively regulates defense against blast infection. MoNLE1 promotes the nuclear migration and degradation of OsPUX8B.2 and disturbs its association with OsBHT. Given the high conservation of MoNLE1 among fungal isolates, plants with broad and durable blast resistance might be generated by engineering intracellular proteins resistant to MoNLE1.

Suggested Citation

  • Xuetao Shi & Xin Xie & Yuanwen Guo & Junqi Zhang & Ziwen Gong & Kai Zhang & Jie Mei & Xinyao Xia & Haoxue Xia & Na Ning & Yutao Xiao & Qing Yang & Guo-Liang Wang & Wende Liu, 2024. "A fungal core effector exploits the OsPUX8B.2–OsCDC48-6 module to suppress plant immunity," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46903-7
    DOI: 10.1038/s41467-024-46903-7
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    References listed on IDEAS

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