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TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity

Author

Listed:
  • Yongliang Zhang

    (University of California, Davis
    China Agricultural University)

  • Gaoyuan Song

    (Iowa State University)

  • Neeraj K. Lal

    (University of California, Davis)

  • Ugrappa Nagalakshmi

    (University of California, Davis)

  • Yuanyuan Li

    (University of California, Davis)

  • Wenjie Zheng

    (University of California, Davis)

  • Pin-jui Huang

    (University of California, Davis)

  • Tess C. Branon

    (Stanford University
    Stanford University
    Stanford University
    Massachusetts Institute of Technology)

  • Alice Y. Ting

    (Stanford University
    Stanford University
    Stanford University
    Chan Zuckerberg Biohub)

  • Justin W. Walley

    (Iowa State University)

  • Savithramma P. Dinesh-Kumar

    (University of California, Davis)

Abstract

Nucleotide-binding leucine-rich repeat (NLR) immune receptors play a critical role in defence against pathogens in plants and animals. However, we know very little about NLR-interacting proteins and the mechanisms that regulate NLR levels. Here, we used proximity labeling (PL) to identify the proteome proximal to N, which is an NLR that confers resistance to Tobacco mosaic virus (TMV). Evaluation of different PL methods indicated that TurboID-based PL provides more efficient levels of biotinylation than BioID and BioID2 in plants. TurboID-based PL of N followed by quantitative proteomic analysis and genetic screening revealed multiple regulators of N-mediated immunity. Interestingly, a putative E3 ubiquitin ligase, UBR7, directly interacts with the TIR domain of N. UBR7 downregulation leads to an increased amount of N protein and enhanced TMV resistance. TMV-p50 effector disrupts the N-UBR7 interaction and relieves negative regulation of N. These findings demonstrate the utility of TurboID-based PL in plants and the N-interacting proteins we identified enhance our understanding of the mechanisms underlying NLR regulation.

Suggested Citation

  • Yongliang Zhang & Gaoyuan Song & Neeraj K. Lal & Ugrappa Nagalakshmi & Yuanyuan Li & Wenjie Zheng & Pin-jui Huang & Tess C. Branon & Alice Y. Ting & Justin W. Walley & Savithramma P. Dinesh-Kumar, 2019. "TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity," Nature Communications, Nature, vol. 10(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11202-z
    DOI: 10.1038/s41467-019-11202-z
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    Cited by:

    1. Xuetao Shi & Xin Xie & Yuanwen Guo & Junqi Zhang & Ziwen Gong & Kai Zhang & Jie Mei & Xinyao Xia & Haoxue Xia & Na Ning & Yutao Xiao & Qing Yang & Guo-Liang Wang & Wende Liu, 2024. "A fungal core effector exploits the OsPUX8B.2–OsCDC48-6 module to suppress plant immunity," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Zongyu Gao & Dingliang Zhang & Xiaoling Wang & Xin Zhang & Zhiyan Wen & Qianshen Zhang & Dawei Li & Savithramma P. Dinesh-Kumar & Yongliang Zhang, 2022. "Coat proteins of necroviruses target 14-3-3a to subvert MAPKKKα-mediated antiviral immunity in plants," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    3. Xue-Ming Wu & Bo-Sen Zhang & Yun-Long Zhao & Hua-Wei Wu & Feng Gao & Jie Zhang & Jian-Hua Zhao & Hui-Shan Guo, 2023. "DeSUMOylation of a Verticillium dahliae enolase facilitates virulence by derepressing the expression of the effector VdSCP8," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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