IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-48313-1.html
   My bibliography  Save this article

Receptor-like cytoplasmic kinases of different subfamilies differentially regulate SOBIR1/BAK1-mediated immune responses in Nicotiana benthamiana

Author

Listed:
  • Wen R. H. Huang

    (Wageningen University
    University of East Anglia)

  • Ciska Braam

    (Wageningen University)

  • Carola Kretschmer

    (Martin Luther University Halle-Wittenberg)

  • Sergio Landeo Villanueva

    (Wageningen University)

  • Huan Liu

    (Wageningen University)

  • Filiz Ferik

    (Martin Luther University Halle-Wittenberg)

  • Aranka M. Burgh

    (Wageningen University
    Wageningen University & Research)

  • Sjef Boeren

    (Wageningen University and Research)

  • Jinbin Wu

    (Wageningen University
    Nanjing Agricultural University)

  • Lisha Zhang

    (Eberhard Karls University Tübingen)

  • Thorsten Nürnberger

    (Eberhard Karls University Tübingen)

  • Yulu Wang

    (Chinese Academy of Agricultural Sciences)

  • Michael F. Seidl

    (Utrecht University)

  • Edouard Evangelisti

    (Wageningen University
    Institut Sophia Agrobiotech (ISA))

  • Johannes Stuttmann

    (Martin Luther University Halle-Wittenberg
    LEMiRE (Microbial Ecology of the Rhizosphere))

  • Matthieu H. A. J. Joosten

    (Wageningen University)

Abstract

Cell-surface receptors form the front line of plant immunity. The leucine-rich repeat (LRR)-receptor-like kinases SOBIR1 and BAK1 are required for the functionality of the tomato LRR-receptor-like protein Cf-4, which detects the secreted effector Avr4 of the pathogenic fungus Fulvia fulva. Here, we show that the kinase domains of SOBIR1 and BAK1 directly phosphorylate each other and that residues Thr522 and Tyr469 of the kinase domain of Nicotiana benthamiana SOBIR1 are required for its kinase activity and for interacting with signalling partners, respectively. By knocking out multiple genes belonging to different receptor-like cytoplasmic kinase (RLCK)-VII subfamilies in N. benthamiana:Cf-4, we show that members of RLCK-VII-6, −7, and −8 differentially regulate the Avr4/Cf-4-triggered biphasic burst of reactive oxygen species. In addition, members of RLCK-VII-7 play an essential role in resistance against the oomycete pathogen Phytophthora palmivora. Our study provides molecular evidence for the specific roles of RLCKs downstream of SOBIR1/BAK1-containing immune complexes.

Suggested Citation

  • Wen R. H. Huang & Ciska Braam & Carola Kretschmer & Sergio Landeo Villanueva & Huan Liu & Filiz Ferik & Aranka M. Burgh & Sjef Boeren & Jinbin Wu & Lisha Zhang & Thorsten Nürnberger & Yulu Wang & Mich, 2024. "Receptor-like cytoplasmic kinases of different subfamilies differentially regulate SOBIR1/BAK1-mediated immune responses in Nicotiana benthamiana," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48313-1
    DOI: 10.1038/s41467-024-48313-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-48313-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-48313-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Rory N. Pruitt & Federica Locci & Friederike Wanke & Lisha Zhang & Svenja C. Saile & Anna Joe & Darya Karelina & Chenlei Hua & Katja Fröhlich & Wei-Lin Wan & Meijuan Hu & Shaofei Rao & Sara C. Stolze , 2021. "The EDS1–PAD4–ADR1 node mediates Arabidopsis pattern-triggered immunity," Nature, Nature, vol. 598(7881), pages 495-499, October.
    2. Yan Wang & Yuanpeng Xu & Yujing Sun & Huibin Wang & Jiaming Qi & Bowen Wan & Wenwu Ye & Yachun Lin & Yuanyuan Shao & Suomeng Dong & Brett M. Tyler & Yuanchao Wang, 2018. "Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    3. Yongliang Zhang & Gaoyuan Song & Neeraj K. Lal & Ugrappa Nagalakshmi & Yuanyuan Li & Wenjie Zheng & Pin-jui Huang & Tess C. Branon & Alice Y. Ting & Justin W. Walley & Savithramma P. Dinesh-Kumar, 2019. "TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity," Nature Communications, Nature, vol. 10(1), pages 1-17, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Li Fan & Katja Fröhlich & Eric Melzer & Rory N. Pruitt & Isabell Albert & Lisha Zhang & Anna Joe & Chenlei Hua & Yanyue Song & Markus Albert & Sang-Tae Kim & Detlef Weigel & Cyril Zipfel & Eunyoung Ch, 2022. "Genotyping-by-sequencing-based identification of Arabidopsis pattern recognition receptor RLP32 recognizing proteobacterial translation initiation factor IF1," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Xue-Ming Wu & Bo-Sen Zhang & Yun-Long Zhao & Hua-Wei Wu & Feng Gao & Jie Zhang & Jian-Hua Zhao & Hui-Shan Guo, 2023. "DeSUMOylation of a Verticillium dahliae enolase facilitates virulence by derepressing the expression of the effector VdSCP8," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. Xiaozhen Zhao & Yiming Wang & Bingqin Yuan & Hanxi Zhao & Yujie Wang & Zheng Tan & Zhiyuan Wang & Huijun Wu & Gang Li & Wei Song & Ravi Gupta & Kenichi Tsuda & Zhonghua Ma & Xuewen Gao & Qin Gu, 2024. "Temporally-coordinated bivalent histone modifications of BCG1 enable fungal invasion and immune evasion," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    4. Zongyu Gao & Dingliang Zhang & Xiaoling Wang & Xin Zhang & Zhiyan Wen & Qianshen Zhang & Dawei Li & Savithramma P. Dinesh-Kumar & Yongliang Zhang, 2022. "Coat proteins of necroviruses target 14-3-3a to subvert MAPKKKα-mediated antiviral immunity in plants," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    5. Jinyi Zhu & Qian Qiao & Yujing Sun & Yuanpeng Xu & Haidong Shu & Zhichao Zhang & Fan Liu & Haonan Wang & Wenwu Ye & Suomeng Dong & Yan Wang & Zhenchuan Ma & Yuanchao Wang, 2023. "Divergent sequences of tetraspanins enable plants to specifically recognize microbe-derived extracellular vesicles," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    6. Xuetao Shi & Xin Xie & Yuanwen Guo & Junqi Zhang & Ziwen Gong & Kai Zhang & Jie Mei & Xinyao Xia & Haoxue Xia & Na Ning & Yutao Xiao & Qing Yang & Guo-Liang Wang & Wende Liu, 2024. "A fungal core effector exploits the OsPUX8B.2–OsCDC48-6 module to suppress plant immunity," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    7. Shen Huang & Chunli Wang & Zixuan Ding & Yaqian Zhao & Jing Dai & Jia Li & Haining Huang & Tongkai Wang & Min Zhu & Mingfeng Feng & Yinghua Ji & Zhongkai Zhang & Xiaorong Tao, 2024. "A plant NLR receptor employs ABA central regulator PP2C-SnRK2 to activate antiviral immunity," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    8. Zhihuan Tao & Lin Zhu & Haichao Li & Bo Sun & Xue Liu & Dayong Li & Wenli Hu & Shanshan Wang & Xuexia Miao & Zhenying Shi, 2024. "ACL1-ROC4/5 complex reveals a common mechanism in rice response to brown planthopper infestation and drought," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    9. Frederickson Entila & Xiaowei Han & Akira Mine & Paul Schulze-Lefert & Kenichi Tsuda, 2024. "Commensal lifestyle regulated by a negative feedback loop between Arabidopsis ROS and the bacterial T2SS," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    10. Yuankun Yang & Christina E. Steidele & Clemens Rössner & Birgit Löffelhardt & Dagmar Kolb & Thomas Leisen & Weiguo Zhang & Christina Ludwig & Georg Felix & Michael F. Seidl & Annette Becker & Thorsten, 2023. "Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48313-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.