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Receptor-like cytoplasmic kinases of different subfamilies differentially regulate SOBIR1/BAK1-mediated immune responses in Nicotiana benthamiana

Author

Listed:
  • Wen R. H. Huang

    (Wageningen University
    University of East Anglia)

  • Ciska Braam

    (Wageningen University)

  • Carola Kretschmer

    (Martin Luther University Halle-Wittenberg)

  • Sergio Landeo Villanueva

    (Wageningen University)

  • Huan Liu

    (Wageningen University)

  • Filiz Ferik

    (Martin Luther University Halle-Wittenberg)

  • Aranka M. Burgh

    (Wageningen University
    Wageningen University & Research)

  • Sjef Boeren

    (Wageningen University and Research)

  • Jinbin Wu

    (Wageningen University
    Nanjing Agricultural University)

  • Lisha Zhang

    (Eberhard Karls University Tübingen)

  • Thorsten Nürnberger

    (Eberhard Karls University Tübingen)

  • Yulu Wang

    (Chinese Academy of Agricultural Sciences)

  • Michael F. Seidl

    (Utrecht University)

  • Edouard Evangelisti

    (Wageningen University
    Institut Sophia Agrobiotech (ISA))

  • Johannes Stuttmann

    (Martin Luther University Halle-Wittenberg
    LEMiRE (Microbial Ecology of the Rhizosphere))

  • Matthieu H. A. J. Joosten

    (Wageningen University)

Abstract

Cell-surface receptors form the front line of plant immunity. The leucine-rich repeat (LRR)-receptor-like kinases SOBIR1 and BAK1 are required for the functionality of the tomato LRR-receptor-like protein Cf-4, which detects the secreted effector Avr4 of the pathogenic fungus Fulvia fulva. Here, we show that the kinase domains of SOBIR1 and BAK1 directly phosphorylate each other and that residues Thr522 and Tyr469 of the kinase domain of Nicotiana benthamiana SOBIR1 are required for its kinase activity and for interacting with signalling partners, respectively. By knocking out multiple genes belonging to different receptor-like cytoplasmic kinase (RLCK)-VII subfamilies in N. benthamiana:Cf-4, we show that members of RLCK-VII-6, −7, and −8 differentially regulate the Avr4/Cf-4-triggered biphasic burst of reactive oxygen species. In addition, members of RLCK-VII-7 play an essential role in resistance against the oomycete pathogen Phytophthora palmivora. Our study provides molecular evidence for the specific roles of RLCKs downstream of SOBIR1/BAK1-containing immune complexes.

Suggested Citation

  • Wen R. H. Huang & Ciska Braam & Carola Kretschmer & Sergio Landeo Villanueva & Huan Liu & Filiz Ferik & Aranka M. Burgh & Sjef Boeren & Jinbin Wu & Lisha Zhang & Thorsten Nürnberger & Yulu Wang & Mich, 2024. "Receptor-like cytoplasmic kinases of different subfamilies differentially regulate SOBIR1/BAK1-mediated immune responses in Nicotiana benthamiana," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48313-1
    DOI: 10.1038/s41467-024-48313-1
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    References listed on IDEAS

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    3. Yongliang Zhang & Gaoyuan Song & Neeraj K. Lal & Ugrappa Nagalakshmi & Yuanyuan Li & Wenjie Zheng & Pin-jui Huang & Tess C. Branon & Alice Y. Ting & Justin W. Walley & Savithramma P. Dinesh-Kumar, 2019. "TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity," Nature Communications, Nature, vol. 10(1), pages 1-17, December.
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