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Proximity labeling proteomics reveals critical regulators for inner nuclear membrane protein degradation in plants

Author

Listed:
  • Aobo Huang

    (Tsinghua University)

  • Yu Tang

    (University of California)

  • Xuetao Shi

    (Tsinghua University)

  • Min Jia

    (University of California)

  • Jinheng Zhu

    (Tsinghua University)

  • Xiaohan Yan

    (Tsinghua University)

  • Huiqin Chen

    (Tsinghua University)

  • Yangnan Gu

    (University of California
    University of California)

Abstract

The inner nuclear membrane (INM) selectively accumulates proteins that are essential for nuclear functions; however, overaccumulation of INM proteins results in a range of rare genetic disorders. So far, little is known about how defective, mislocalized, or abnormally accumulated membrane proteins are actively removed from the INM, especially in plants and animals. Here, via analysis of a proximity-labeling proteomic profile of INM-associated proteins in Arabidopsis, we identify critical components for an INM protein degradation pathway. We show that this pathway relies on the CDC48 complex for INM protein extraction and 26S proteasome for subsequent protein degradation. Moreover, we show that CDC48 at the INM may be regulated by a subgroup of PUX proteins, which determine the substrate specificity or affect the ATPase activity of CDC48. These PUX proteins specifically associate with the nucleoskeleton underneath the INM and physically interact with CDC48 proteins to negatively regulate INM protein degradation in plants.

Suggested Citation

  • Aobo Huang & Yu Tang & Xuetao Shi & Min Jia & Jinheng Zhu & Xiaohan Yan & Huiqin Chen & Yangnan Gu, 2020. "Proximity labeling proteomics reveals critical regulators for inner nuclear membrane protein degradation in plants," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16744-1
    DOI: 10.1038/s41467-020-16744-1
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    Cited by:

    1. Xuetao Shi & Xin Xie & Yuanwen Guo & Junqi Zhang & Ziwen Gong & Kai Zhang & Jie Mei & Xinyao Xia & Haoxue Xia & Na Ning & Yutao Xiao & Qing Yang & Guo-Liang Wang & Wende Liu, 2024. "A fungal core effector exploits the OsPUX8B.2–OsCDC48-6 module to suppress plant immunity," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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