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Plant immunity suppression by an exo-β-1,3-glucanase and an elongation factor 1α of the rice blast fungus

Author

Listed:
  • Hang Liu

    (China Agricultural University)

  • Xunli Lu

    (China Agricultural University)

  • Mengfei Li

    (China Agricultural University)

  • Zhiqin Lun

    (China Agricultural University)

  • Xia Yan

    (University of East Anglia, Norwich Research Park)

  • Changfa Yin

    (China Agricultural University)

  • Guixin Yuan

    (China Agricultural University)

  • Xingbin Wang

    (China Agricultural University)

  • Ning Liu

    (China Agricultural University)

  • Di Liu

    (China Agricultural University)

  • Mian Wu

    (China Agricultural University)

  • Ziluolong Luo

    (China Agricultural University)

  • Yan Zhang

    (China Agricultural University)

  • Vijai Bhadauria

    (China Agricultural University)

  • Jun Yang

    (China Agricultural University)

  • Nicholas J. Talbot

    (University of East Anglia, Norwich Research Park)

  • You-Liang Peng

    (China Agricultural University)

Abstract

Fungal cell walls undergo continual remodeling that generates β-1,3-glucan fragments as products of endo-glycosyl hydrolases (GHs), which can be recognized as pathogen-associated molecular patterns (PAMPs) and trigger plant immune responses. How fungal pathogens suppress those responses is often poorly understood. Here, we study mechanisms underlying the suppression of β-1,3-glucan-triggered plant immunity by the blast fungus Magnaporthe oryzae. We show that an exo-β-1,3-glucanase of the GH17 family, named Ebg1, is important for fungal cell wall integrity and virulence of M. oryzae. Ebg1 can hydrolyze β-1,3-glucan and laminarin into glucose, thus suppressing β-1,3-glucan-triggered plant immunity. However, in addition, Ebg1 seems to act as a PAMP, independent of its hydrolase activity. This Ebg1-induced immunity appears to be dampened by the secretion of an elongation factor 1 alpha protein (EF1α), which interacts and co-localizes with Ebg1 in the apoplast. Future work is needed to understand the mechanisms behind Ebg1-induced immunity and its suppression by EF1α.

Suggested Citation

  • Hang Liu & Xunli Lu & Mengfei Li & Zhiqin Lun & Xia Yan & Changfa Yin & Guixin Yuan & Xingbin Wang & Ning Liu & Di Liu & Mian Wu & Ziluolong Luo & Yan Zhang & Vijai Bhadauria & Jun Yang & Nicholas J. , 2023. "Plant immunity suppression by an exo-β-1,3-glucanase and an elongation factor 1α of the rice blast fungus," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41175-z
    DOI: 10.1038/s41467-023-41175-z
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    1. Kathryn Tunyasuvunakool & Jonas Adler & Zachary Wu & Tim Green & Michal Zielinski & Augustin Žídek & Alex Bridgland & Andrew Cowie & Clemens Meyer & Agata Laydon & Sameer Velankar & Gerard J. Kleywegt, 2021. "Highly accurate protein structure prediction for the human proteome," Nature, Nature, vol. 596(7873), pages 590-596, August.
    2. John Jumper & Richard Evans & Alexander Pritzel & Tim Green & Michael Figurnov & Olaf Ronneberger & Kathryn Tunyasuvunakool & Russ Bates & Augustin Žídek & Anna Potapenko & Alex Bridgland & Clemens Me, 2021. "Highly accurate protein structure prediction with AlphaFold," Nature, Nature, vol. 596(7873), pages 583-589, August.
    3. Tiffany A. Reese & Hong-Erh Liang & Andrew M. Tager & Andrew D. Luster & Nico Van Rooijen & David Voehringer & Richard M. Locksley, 2007. "Chitin induces accumulation in tissue of innate immune cells associated with allergy," Nature, Nature, vol. 447(7140), pages 92-96, May.
    4. Ralph A. Dean & Nicholas J. Talbot & Daniel J. Ebbole & Mark L. Farman & Thomas K. Mitchell & Marc J. Orbach & Michael Thon & Resham Kulkarni & Jin-Rong Xu & Huaqin Pan & Nick D. Read & Yong-Hwan Lee , 2005. "The genome sequence of the rice blast fungus Magnaporthe grisea," Nature, Nature, vol. 434(7036), pages 980-986, April.
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