IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-41013-2.html
   My bibliography  Save this article

Functionalization and higher-order organization of liposomes with DNA nanostructures

Author

Listed:
  • Zhao Zhang

    (University of Wisconsin−Madison)

  • Zhaomeng Feng

    (University of Wisconsin−Madison)

  • Xiaowei Zhao

    (Janelia Research Campus, 19700 Helix Drive)

  • Dominique Jean

    (Janelia Research Campus, 19700 Helix Drive)

  • Zhiheng Yu

    (Janelia Research Campus, 19700 Helix Drive)

  • Edwin R. Chapman

    (University of Wisconsin−Madison)

Abstract

Small unilamellar vesicles (SUVs) are indispensable model membranes, organelle mimics, and drug and vaccine carriers. However, the lack of robust techniques to functionalize or organize preformed SUVs limits their applications. Here we use DNA nanostructures to coat, cluster, and pattern sub-100-nm liposomes, generating distance-controlled vesicle networks, strings and dimers, among other configurations. The DNA coating also enables attachment of proteins to liposomes, and temporal control of membrane fusion driven by SNARE protein complexes. Such a convenient and versatile method of engineering premade vesicles both structurally and functionally is highly relevant to bottom-up biology and targeted delivery.

Suggested Citation

  • Zhao Zhang & Zhaomeng Feng & Xiaowei Zhao & Dominique Jean & Zhiheng Yu & Edwin R. Chapman, 2023. "Functionalization and higher-order organization of liposomes with DNA nanostructures," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41013-2
    DOI: 10.1038/s41467-023-41013-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-41013-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-41013-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Henri G. Franquelim & Alena Khmelinskaia & Jean-Philippe Sobczak & Hendrik Dietz & Petra Schwille, 2018. "Membrane sculpting by curved DNA origami scaffolds," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    2. Bernard Yurke & Andrew J. Turberfield & Allen P. Mills & Friedrich C. Simmel & Jennifer L. Neumann, 2000. "A DNA-fuelled molecular machine made of DNA," Nature, Nature, vol. 406(6796), pages 605-608, August.
    3. Rasmus P. Thomsen & Mette Galsgaard Malle & Anders Hauge Okholm & Swati Krishnan & Søren S.-R. Bohr & Rasmus Schøler Sørensen & Oliver Ries & Stefan Vogel & Friedrich C. Simmel & Nikos S. Hatzakis & J, 2019. "A large size-selective DNA nanopore with sensing applications," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    4. Shawn M. Douglas & Hendrik Dietz & Tim Liedl & Björn Högberg & Franziska Graf & William M. Shih, 2009. "Self-assembly of DNA into nanoscale three-dimensional shapes," Nature, Nature, vol. 459(7245), pages 414-418, May.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Swarup Dey & Adam Dorey & Leeza Abraham & Yongzheng Xing & Irene Zhang & Fei Zhang & Stefan Howorka & Hao Yan, 2022. "A reversibly gated protein-transporting membrane channel made of DNA," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Vishal Maingi & Zhao Zhang & Chris Thachuk & Namita Sarraf & Edwin R. Chapman & Paul W. K. Rothemund, 2023. "Digital nanoreactors to control absolute stoichiometry and spatiotemporal behavior of DNA receptors within lipid bilayers," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    3. Ioanna Smyrlaki & Ferenc Fördős & Iris Rocamonde-Lago & Yang Wang & Boxuan Shen & Antonio Lentini & Vincent C. Luca & Björn Reinius & Ana I. Teixeira & Björn Högberg, 2024. "Soluble and multivalent Jag1 DNA origami nanopatterns activate Notch without pulling force," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    4. Sungwook Woo & Sinem K. Saka & Feng Xuan & Peng Yin, 2024. "Molecular robotic agents that survey molecular landscapes for information retrieval," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    5. Jessica A. Kretzmann & Anna Liedl & Alba Monferrer & Volodymyr Mykhailiuk & Samuel Beerkens & Hendrik Dietz, 2023. "Gene-encoding DNA origami for mammalian cell expression," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    6. Molly F. Parsons & Matthew F. Allan & Shanshan Li & Tyson R. Shepherd & Sakul Ratanalert & Kaiming Zhang & Krista M. Pullen & Wah Chiu & Silvi Rouskin & Mark Bathe, 2023. "3D RNA-scaffolded wireframe origami," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    7. Andreas Walbrun & Tianhe Wang & Michael Matthies & Petr Šulc & Friedrich C. Simmel & Matthias Rief, 2024. "Single-molecule force spectroscopy of toehold-mediated strand displacement," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    8. Martina F. Ober & Anna Baptist & Lea Wassermann & Amelie Heuer-Jungemann & Bert Nickel, 2022. "In situ small-angle X-ray scattering reveals strong condensation of DNA origami during silicification," Nature Communications, Nature, vol. 13(1), pages 1-7, December.
    9. Francis Schuknecht & Karol Kołątaj & Michael Steinberger & Tim Liedl & Theobald Lohmueller, 2023. "Accessible hotspots for single-protein SERS in DNA-origami assembled gold nanorod dimers with tip-to-tip alignment," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    10. A. Mills & N. Aissaoui & D. Maurel & J. Elezgaray & F. Morvan & J. J. Vasseur & E. Margeat & R. B. Quast & J. Lai Kee-Him & N. Saint & C. Benistant & A. Nord & F. Pedaci & G. Bellot, 2022. "A modular spring-loaded actuator for mechanical activation of membrane proteins," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    11. Tai-Yin Chiu & Hui-Ju K Chiang & Ruei-Yang Huang & Jie-Hong R Jiang & François Fages, 2015. "Synthesizing Configurable Biochemical Implementation of Linear Systems from Their Transfer Function Specifications," PLOS ONE, Public Library of Science, vol. 10(9), pages 1-27, September.
    12. Solveig G. Schmidt & Mette Galsgaard Malle & Anne Kathrine Nielsen & Søren S.-R. Bohr & Ciara F. Pugh & Jeppe C. Nielsen & Ida H. Poulsen & Kasper D. Rand & Nikos S. Hatzakis & Claus J. Loland, 2022. "The dopamine transporter antiports potassium to increase the uptake of dopamine," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    13. Ehsan Akbari & Melika Shahhosseini & Ariel Robbins & Michael G. Poirier & Jonathan W. Song & Carlos E. Castro, 2022. "Low cost and massively parallel force spectroscopy with fluid loading on a chip," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    14. Ferdinand Greiss & Nicolas Lardon & Leonie Schütz & Yoav Barak & Shirley S. Daube & Elmar Weinhold & Vincent Noireaux & Roy Bar-Ziv, 2024. "A genetic circuit on a single DNA molecule as an autonomous dissipative nanodevice," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    15. Chi Chen & Xingfei Wei & Molly F. Parsons & Jiajia Guo & James L. Banal & Yinong Zhao & Madelyn N. Scott & Gabriela S. Schlau-Cohen & Rigoberto Hernandez & Mark Bathe, 2022. "Nanoscale 3D spatial addressing and valence control of quantum dots using wireframe DNA origami," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    16. Xiaoming Liu & Fengyu Liu & Hemani Chhabra & Christopher Maffeo & Zhuo Chen & Qiang Huang & Aleksei Aksimentiev & Tatsuo Arai, 2024. "A lumen-tunable triangular DNA nanopore for molecular sensing and cross-membrane transport," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    17. Eva Bertosin & Christopher M. Maffeo & Thomas Drexler & Maximilian N. Honemann & Aleksei Aksimentiev & Hendrik Dietz, 2021. "A nanoscale reciprocating rotary mechanism with coordinated mobility control," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    18. Linlin Tang & Zhijin Tian & Jin Cheng & Yijing Zhang & Yongxiu Song & Yan Liu & Jinghao Wang & Pengfei Zhang & Yonggang Ke & Friedrich C. Simmel & Jie Song, 2023. "Circular single-stranded DNA as switchable vector for gene expression in mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    19. Jae Young Lee & Heeyuen Koh & Do-Nyun Kim, 2023. "A computational model for structural dynamics and reconfiguration of DNA assemblies," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    20. Hong Kang & Yuexuan Yang & Bryan Wei, 2024. "Synthetic molecular switches driven by DNA-modifying enzymes," Nature Communications, Nature, vol. 15(1), pages 1-9, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41013-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.