IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-36601-1.html
   My bibliography  Save this article

Gene-encoding DNA origami for mammalian cell expression

Author

Listed:
  • Jessica A. Kretzmann

    (Technical University of Munich
    Technical University of Munich)

  • Anna Liedl

    (Technical University of Munich
    Technical University of Munich)

  • Alba Monferrer

    (Technical University of Munich
    Technical University of Munich)

  • Volodymyr Mykhailiuk

    (Technical University of Munich
    Technical University of Munich)

  • Samuel Beerkens

    (Technical University of Munich
    Technical University of Munich)

  • Hendrik Dietz

    (Technical University of Munich
    Technical University of Munich)

Abstract

DNA origami may enable more versatile gene delivery applications through its ability to create custom nanoscale objects with specific targeting, cell-invading, and intracellular effector functionalities. Toward this goal here we describe the expression of genes folded in DNA origami objects delivered to mammalian cells. Genes readily express from custom-sequence single-strand scaffolds folded within DNA origami objects, provided that the objects can denature in the cell. We demonstrate enhanced gene expression efficiency by including and tuning multiple functional sequences and structures, including virus-inspired inverted-terminal repeat-like (ITR) hairpin motifs upstream or flanking the expression cassette. We describe gene-encoding DNA origami bricks that assemble into multimeric objects to enable stoichiometrically controlled co-delivery and expression of multiple genes in the same cells. Our work provides a framework for exploiting DNA origami for gene delivery applications.

Suggested Citation

  • Jessica A. Kretzmann & Anna Liedl & Alba Monferrer & Volodymyr Mykhailiuk & Samuel Beerkens & Hendrik Dietz, 2023. "Gene-encoding DNA origami for mammalian cell expression," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36601-1
    DOI: 10.1038/s41467-023-36601-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-36601-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-36601-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Nicholas S. McCarty & Alicia E. Graham & Lucie Studená & Rodrigo Ledesma-Amaro, 2020. "Multiplexed CRISPR technologies for gene editing and transcriptional regulation," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    2. Florian Praetorius & Benjamin Kick & Karl L. Behler & Maximilian N. Honemann & Dirk Weuster-Botz & Hendrik Dietz, 2017. "Biotechnological mass production of DNA origami," Nature, Nature, vol. 552(7683), pages 84-87, December.
    3. Karen Bulaklak & Charles A. Gersbach, 2020. "The once and future gene therapy," Nature Communications, Nature, vol. 11(1), pages 1-4, December.
    4. Henri G. Franquelim & Alena Khmelinskaia & Jean-Philippe Sobczak & Hendrik Dietz & Petra Schwille, 2018. "Membrane sculpting by curved DNA origami scaffolds," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    5. Klaus F. Wagenbauer & Christian Sigl & Hendrik Dietz, 2017. "Gigadalton-scale shape-programmable DNA assemblies," Nature, Nature, vol. 552(7683), pages 78-83, December.
    6. Shawn M. Douglas & Hendrik Dietz & Tim Liedl & Björn Högberg & Franziska Graf & William M. Shih, 2009. "Self-assembly of DNA into nanoscale three-dimensional shapes," Nature, Nature, vol. 459(7245), pages 414-418, May.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Linlin Tang & Zhijin Tian & Jin Cheng & Yijing Zhang & Yongxiu Song & Yan Liu & Jinghao Wang & Pengfei Zhang & Yonggang Ke & Friedrich C. Simmel & Jie Song, 2023. "Circular single-stranded DNA as switchable vector for gene expression in mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Zhijin Tian & Dandan Shao & Linlin Tang & Zhen Li & Qian Chen & Yongxiu Song & Tao Li & Friedrich C. Simmel & Jie Song, 2024. "Circular single-stranded DNA as a programmable vector for gene regulation in cell-free protein expression systems," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yahong Chen & Chaoyong Yang & Zhi Zhu & Wei Sun, 2022. "Suppressing high-dimensional crystallographic defects for ultra-scaled DNA arrays," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Vishal Maingi & Zhao Zhang & Chris Thachuk & Namita Sarraf & Edwin R. Chapman & Paul W. K. Rothemund, 2023. "Digital nanoreactors to control absolute stoichiometry and spatiotemporal behavior of DNA receptors within lipid bilayers," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    3. Eva Bertosin & Christopher M. Maffeo & Thomas Drexler & Maximilian N. Honemann & Aleksei Aksimentiev & Hendrik Dietz, 2021. "A nanoscale reciprocating rotary mechanism with coordinated mobility control," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    4. Zhao Zhang & Zhaomeng Feng & Xiaowei Zhao & Dominique Jean & Zhiheng Yu & Edwin R. Chapman, 2023. "Functionalization and higher-order organization of liposomes with DNA nanostructures," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    5. Molly F. Parsons & Matthew F. Allan & Shanshan Li & Tyson R. Shepherd & Sakul Ratanalert & Kaiming Zhang & Krista M. Pullen & Wah Chiu & Silvi Rouskin & Mark Bathe, 2023. "3D RNA-scaffolded wireframe origami," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    6. Martina F. Ober & Anna Baptist & Lea Wassermann & Amelie Heuer-Jungemann & Bert Nickel, 2022. "In situ small-angle X-ray scattering reveals strong condensation of DNA origami during silicification," Nature Communications, Nature, vol. 13(1), pages 1-7, December.
    7. Qichen Yuan & Xue Gao, 2022. "Multiplex base- and prime-editing with drive-and-process CRISPR arrays," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    8. William M. Shaw & Lucie Studená & Kyler Roy & Piotr Hapeta & Nicholas S. McCarty & Alicia E. Graham & Tom Ellis & Rodrigo Ledesma-Amaro, 2022. "Inducible expression of large gRNA arrays for multiplexed CRISPRai applications," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    9. Francis Schuknecht & Karol Kołątaj & Michael Steinberger & Tim Liedl & Theobald Lohmueller, 2023. "Accessible hotspots for single-protein SERS in DNA-origami assembled gold nanorod dimers with tip-to-tip alignment," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    10. A. Mills & N. Aissaoui & D. Maurel & J. Elezgaray & F. Morvan & J. J. Vasseur & E. Margeat & R. B. Quast & J. Lai Kee-Him & N. Saint & C. Benistant & A. Nord & F. Pedaci & G. Bellot, 2022. "A modular spring-loaded actuator for mechanical activation of membrane proteins," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    11. Peter N. Ciaccia & Zhuobin Liang & Anabel Y. Schweitzer & Eli Metzner & Farren J. Isaacs, 2024. "Enhanced eMAGE applied to identify genetic factors of nuclear hormone receptor dysfunction via combinatorial gene editing," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    12. Yuting Chen & Eriona Hysolli & Anlu Chen & Stephen Casper & Songlei Liu & Kevin Yang & Chenli Liu & George Church, 2022. "Multiplex base editing to convert TAG into TAA codons in the human genome," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    13. Sally Wang & Chen-Yu Chen & John R. Rzasa & Chen-Yu Tsao & Jinyang Li & Eric VanArsdale & Eunkyoung Kim & Fauziah Rahma Zakaria & Gregory F. Payne & William E. Bentley, 2023. "Redox-enabled electronic interrogation and feedback control of hierarchical and networked biological systems," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    14. Guanhua Xun & Zhixin Zhu & Nilmani Singh & Jingxia Lu & Piyush K. Jain & Huimin Zhao, 2024. "Harnessing noncanonical crRNA for highly efficient genome editing," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    15. Ioanna Smyrlaki & Ferenc Fördős & Iris Rocamonde-Lago & Yang Wang & Boxuan Shen & Antonio Lentini & Vincent C. Luca & Björn Reinius & Ana I. Teixeira & Björn Högberg, 2024. "Soluble and multivalent Jag1 DNA origami nanopatterns activate Notch without pulling force," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    16. Chi Chen & Xingfei Wei & Molly F. Parsons & Jiajia Guo & James L. Banal & Yinong Zhao & Madelyn N. Scott & Gabriela S. Schlau-Cohen & Rigoberto Hernandez & Mark Bathe, 2022. "Nanoscale 3D spatial addressing and valence control of quantum dots using wireframe DNA origami," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    17. Swarup Dey & Adam Dorey & Leeza Abraham & Yongzheng Xing & Irene Zhang & Fei Zhang & Stefan Howorka & Hao Yan, 2022. "A reversibly gated protein-transporting membrane channel made of DNA," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    18. Huacheng Li & Xin Xu & Rongcheng Guan & Artur Movsesyan & Zhenni Lu & Qiliang Xu & Ziyun Jiang & Yurong Yang & Majid Khan & Jin Wen & Hongwei Wu & Santiago Moya & Gil Markovich & Huatian Hu & Zhiming , 2024. "Collective chiroptical activity through the interplay of excitonic and charge-transfer effects in localized plasmonic fields," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    19. Linlin Tang & Zhijin Tian & Jin Cheng & Yijing Zhang & Yongxiu Song & Yan Liu & Jinghao Wang & Pengfei Zhang & Yonggang Ke & Friedrich C. Simmel & Jie Song, 2023. "Circular single-stranded DNA as switchable vector for gene expression in mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    20. Jianli Tao & Daniel E. Bauer & Roberto Chiarle, 2023. "Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36601-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.