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Synthetic molecular switches driven by DNA-modifying enzymes

Author

Listed:
  • Hong Kang

    (Tsinghua University
    University of Pennsylvania Perelman School of Medicine)

  • Yuexuan Yang

    (Tsinghua University)

  • Bryan Wei

    (Tsinghua University)

Abstract

Taking inspiration from natural systems, in which molecular switches are ubiquitous in the biochemistry regulatory network, we aim to design and construct synthetic molecular switches driven by DNA-modifying enzymes, such as DNA polymerase and nicking endonuclease. The enzymatic treatments on our synthetic DNA constructs controllably switch ON or OFF the sticky end cohesion and in turn cascade to the structural association or disassociation. Here we showcase the concept in multiple DNA nanostructure systems with robust assembly/disassembly performance. The switch mechanisms are first illustrated in minimalist systems with a few DNA strands. Then the ON/OFF switches are realized in complex DNA lattice and origami systems with designated morphological changes responsive to the specific enzymatic treatments.

Suggested Citation

  • Hong Kang & Yuexuan Yang & Bryan Wei, 2024. "Synthetic molecular switches driven by DNA-modifying enzymes," Nature Communications, Nature, vol. 15(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47742-2
    DOI: 10.1038/s41467-024-47742-2
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    References listed on IDEAS

    as
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