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High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217

Author

Listed:
  • Fenglin Li

    (Thomas Jefferson University)

  • Chun-Feng David Hou

    (Thomas Jefferson University)

  • Ravi K. Lokareddy

    (Thomas Jefferson University)

  • Ruoyu Yang

    (Thomas Jefferson University)

  • Francesca Forti

    (Università degli Studi di Milano)

  • Federica Briani

    (Università degli Studi di Milano)

  • Gino Cingolani

    (Thomas Jefferson University)

Abstract

E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 Å and 4.5 Å resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4.

Suggested Citation

  • Fenglin Li & Chun-Feng David Hou & Ravi K. Lokareddy & Ruoyu Yang & Francesca Forti & Federica Briani & Gino Cingolani, 2023. "High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39756-z
    DOI: 10.1038/s41467-023-39756-z
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    1. Ravi K. Lokareddy & Chun-Feng David Hou & Francesca Forti & Stephano M. Iglesias & Fenglin Li & Mikhail Pavlenok & David S. Horner & Michael Niederweis & Federica Briani & Gino Cingolani, 2024. "Integrative structural analysis of Pseudomonas phage DEV reveals a genome ejection motor," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

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