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Cryo-EM structure of ssDNA bacteriophage ΦCjT23 provides insight into early virus evolution

Author

Listed:
  • Nejc Kejzar

    (University of Helsinki
    University of Cambridge)

  • Elina Laanto

    (University of Helsinki
    University of Jyväskylä)

  • Ilona Rissanen

    (University of Helsinki)

  • Vahid Abrishami

    (University of Helsinki)

  • Muniyandi Selvaraj

    (University of Helsinki
    Norwich Research Park)

  • Sylvain Moineau

    (Université Laval, Québec City)

  • Janne Ravantti

    (University of Helsinki)

  • Lotta-Riina Sundberg

    (University of Jyväskylä)

  • Juha T. Huiskonen

    (University of Helsinki)

Abstract

The origin of viruses remains an open question. While lack of detectable sequence similarity hampers the analysis of distantly related viruses, structural biology investigations of conserved capsid protein structures facilitate the study of distant evolutionary relationships. Here we characterize the lipid-containing ssDNA temperate bacteriophage ΦCjT23, which infects Flavobacterium sp. (Bacteroidetes). We report ΦCjT23-like sequences in the genome of strains belonging to several Flavobacterium species. The virion structure determined by cryogenic electron microscopy reveals similarities to members of the viral kingdom Bamfordvirae that currently consists solely of dsDNA viruses with a major capsid protein composed of two upright β-sandwiches. The minimalistic structure of ΦCjT23 suggests that this phage serves as a model for the last common ancestor between ssDNA and dsDNA viruses in the Bamfordvirae. Both ΦCjT23 and the related phage FLiP infect Flavobacterium species found in several environments, suggesting that these types of viruses have a global distribution and a shared evolutionary origin. Detailed comparisons to related, more complex viruses not only expand our knowledge about this group of viruses but also provide a rare glimpse into early virus evolution.

Suggested Citation

  • Nejc Kejzar & Elina Laanto & Ilona Rissanen & Vahid Abrishami & Muniyandi Selvaraj & Sylvain Moineau & Janne Ravantti & Lotta-Riina Sundberg & Juha T. Huiskonen, 2022. "Cryo-EM structure of ssDNA bacteriophage ΦCjT23 provides insight into early virus evolution," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35123-6
    DOI: 10.1038/s41467-022-35123-6
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    References listed on IDEAS

    as
    1. Kathryn E. Huber & Matthew K. Waldor, 2002. "Filamentous phage integration requires the host recombinases XerC and XerD," Nature, Nature, vol. 417(6889), pages 656-659, June.
    2. Serban L. Ilca & Xiaoyu Sun & Kamel El Omari & Abhay Kotecha & Felix Haas & Frank DiMaio & Jonathan M. Grimes & David I. Stuart & Minna M. Poranen & Juha T. Huiskonen, 2019. "Multiple liquid crystalline geometries of highly compacted nucleic acid in a dsRNA virus," Nature, Nature, vol. 570(7760), pages 252-256, June.
    3. Serban L. Ilca & Abhay Kotecha & Xiaoyu Sun & Minna M. Poranen & David I. Stuart & Juha T. Huiskonen, 2015. "Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes," Nature Communications, Nature, vol. 6(1), pages 1-8, December.
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