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Structure and proposed DNA delivery mechanism of a marine roseophage

Author

Listed:
  • Yang Huang

    (Xiamen University
    Xiamen University)

  • Hui Sun

    (Xiamen University
    Xiamen University)

  • Shuzhen Wei

    (Xiamen University)

  • Lanlan Cai

    (The Hong Kong University of Science and Technology)

  • Liqin Liu

    (Xiamen University
    Xiamen University)

  • Yanan Jiang

    (Xiamen University
    Xiamen University)

  • Jiabao Xin

    (Xiamen University
    Xiamen University)

  • Zhenqin Chen

    (Xiamen University
    Xiamen University)

  • Yuqiong Que

    (Xiamen University
    Xiamen University)

  • Zhibo Kong

    (Xiamen University
    Xiamen University)

  • Tingting Li

    (Xiamen University
    Xiamen University)

  • Hai Yu

    (Xiamen University
    Xiamen University)

  • Jun Zhang

    (Xiamen University
    Xiamen University)

  • Ying Gu

    (Xiamen University
    Xiamen University)

  • Qingbing Zheng

    (Xiamen University
    Xiamen University)

  • Shaowei Li

    (Xiamen University
    Xiamen University)

  • Rui Zhang

    (Xiamen University
    Shenzhen University)

  • Ningshao Xia

    (Xiamen University
    Xiamen University
    Chinese Academy of Medical Sciences)

Abstract

Tailed bacteriophages (order, Caudovirales) account for the majority of all phages. However, the long flexible tail of siphophages hinders comprehensive investigation of the mechanism of viral gene delivery. Here, we report the atomic capsid and in-situ structures of the tail machine of the marine siphophage, vB_DshS-R4C (R4C), which infects Roseobacter. The R4C virion, comprising 12 distinct structural protein components, has a unique five-fold vertex of the icosahedral capsid that allows genome delivery. The specific position and interaction pattern of the tail tube proteins determine the atypical long rigid tail of R4C, and further provide negative charge distribution within the tail tube. A ratchet mechanism assists in DNA transmission, which is initiated by an absorption device that structurally resembles the phage-like particle, RcGTA. Overall, these results provide in-depth knowledge into the intact structure and underlining DNA delivery mechanism for the ecologically important siphophages.

Suggested Citation

  • Yang Huang & Hui Sun & Shuzhen Wei & Lanlan Cai & Liqin Liu & Yanan Jiang & Jiabao Xin & Zhenqin Chen & Yuqiong Que & Zhibo Kong & Tingting Li & Hai Yu & Jun Zhang & Ying Gu & Qingbing Zheng & Shaowei, 2023. "Structure and proposed DNA delivery mechanism of a marine roseophage," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39220-y
    DOI: 10.1038/s41467-023-39220-y
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    References listed on IDEAS

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    Cited by:

    1. Natalia Quinones-Olvera & Siân V. Owen & Lucy M. McCully & Maximillian G. Marin & Eleanor A. Rand & Alice C. Fan & Oluremi J. Martins Dosumu & Kay Paul & Cleotilde E. Sanchez Castaño & Rachel Petherbr, 2024. "Diverse and abundant phages exploit conjugative plasmids," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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