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Architecture of the baculovirus nucleocapsid revealed by cryo-EM

Author

Listed:
  • Xudong Jia

    (Sun Yat-sen University)

  • Yuanzhu Gao

    (Sun Yat-sen University
    Southern University of Science and Technology)

  • Yuxuan Huang

    (Sun Yat-sen University)

  • Linjun Sun

    (Sun Yat-sen University)

  • Siduo Li

    (Sun Yat-sen University)

  • Hongmei Li

    (Sun Yat-sen University)

  • Xueqing Zhang

    (Sun Yat-sen University)

  • Yinyin Li

    (Sun Yat-sen University)

  • Jian He

    (Sun Yat-sen University)

  • Wenbi Wu

    (Sun Yat-sen University)

  • Harikanth Venkannagari

    (McGovern Medical School at the University of Texas Health Science Center)

  • Kai Yang

    (Sun Yat-sen University)

  • Matthew L. Baker

    (McGovern Medical School at the University of Texas Health Science Center)

  • Qinfen Zhang

    (Sun Yat-sen University)

Abstract

Baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) has been widely used as a bioinsecticide and a protein expression vector. Despite their importance, very little is known about the structure of most baculovirus proteins. Here, we show a 3.2 Å resolution structure of helical cylindrical body of the AcMNPV nucleocapsid, composed of VP39, as well as 4.3 Å resolution structures of both the head and the base of the nucleocapsid composed of over 100 protein subunits. AcMNPV VP39 demonstrates some features of the HK97-like fold and utilizes disulfide-bonds and a set of interactions at its C-termini to mediate nucleocapsid assembly and stability. At both ends of the nucleocapsid, the VP39 cylinder is constricted by an outer shell ring composed of proteins AC104, AC142 and AC109. AC101(BV/ODV-C42) and AC144(ODV-EC27) form a C14 symmetric inner layer at both capsid head and base. In the base, these proteins interact with a 7-fold symmetric capsid plug, while a portal-like structure is seen in the central portion of head. Additionally, we propose an application of AlphaFold2 for model building in intermediate resolution density.

Suggested Citation

  • Xudong Jia & Yuanzhu Gao & Yuxuan Huang & Linjun Sun & Siduo Li & Hongmei Li & Xueqing Zhang & Yinyin Li & Jian He & Wenbi Wu & Harikanth Venkannagari & Kai Yang & Matthew L. Baker & Qinfen Zhang, 2023. "Architecture of the baculovirus nucleocapsid revealed by cryo-EM," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43284-1
    DOI: 10.1038/s41467-023-43284-1
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    References listed on IDEAS

    as
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    2. Kathryn Tunyasuvunakool & Jonas Adler & Zachary Wu & Tim Green & Michal Zielinski & Augustin Žídek & Alex Bridgland & Andrew Cowie & Clemens Meyer & Agata Laydon & Sameer Velankar & Gerard J. Kleywegt, 2021. "Highly accurate protein structure prediction for the human proteome," Nature, Nature, vol. 596(7873), pages 590-596, August.
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