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PlzR regulates type IV pili assembly in Pseudomonas aeruginosa via PilZ binding

Author

Listed:
  • Hanne Hendrix

    (KU Leuven)

  • Annabel Itterbeek

    (KU Leuven
    KU Leuven)

  • Hannelore Longin

    (KU Leuven
    KU Leuven)

  • Lize Delanghe

    (KU Leuven)

  • Eveline Vriens

    (KU Leuven)

  • Marta Vallino

    (Institute for Sustainable Plant Protection, National Research Council of Italy, IPSP-CNR Headquarter)

  • Eveline-Marie Lammens

    (KU Leuven)

  • Farhana Haque

    (Texas A&M University)

  • Ahmed Yusuf

    (Texas A&M University)

  • Jean-Paul Noben

    (Hasselt University)

  • Maarten Boon

    (KU Leuven)

  • Matthias D. Koch

    (Texas A&M University)

  • Vera Noort

    (KU Leuven
    Leiden University)

  • Rob Lavigne

    (KU Leuven)

Abstract

Type IV pili (T4P) are thin, flexible filaments exposed on the cell surface of gram-negative bacteria and are involved in pathogenesis-related processes, including cell adsorption, biofilm formation, and twitching motility. Bacteriophages often use these filaments as receptors to infect host cells. Here, we describe the identification of a protein that inhibits T4P assembly in Pseudomonas aeruginosa, discovered during a screen for host factors influencing phage infection. We show that expression of PA2560 (renamed PlzR) in P. aeruginosa inhibits adsorption of T4P-dependent phages. PlzR does this by directly binding the T4P chaperone PilZ, which in turn regulates the ATPase PilB and results in disturbed T4P assembly. As the plzR promoter is induced by cyclic di-GMP, PlzR might play a role in coupling T4P function to levels of this second messenger.

Suggested Citation

  • Hanne Hendrix & Annabel Itterbeek & Hannelore Longin & Lize Delanghe & Eveline Vriens & Marta Vallino & Eveline-Marie Lammens & Farhana Haque & Ahmed Yusuf & Jean-Paul Noben & Maarten Boon & Matthias , 2024. "PlzR regulates type IV pili assembly in Pseudomonas aeruginosa via PilZ binding," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-52732-5
    DOI: 10.1038/s41467-024-52732-5
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    References listed on IDEAS

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    1. Brieuc Van Nieuwenhuyse & Dimitri Van der Linden & Olga Chatzis & Cédric Lood & Jeroen Wagemans & Rob Lavigne & Kaat Schroven & Jan Paeshuyse & Catherine de Magnée & Etienne Sokal & Xavier Stéphenne &, 2022. "Bacteriophage-antibiotic combination therapy against extensively drug-resistant Pseudomonas aeruginosa infection to allow liver transplantation in a toddler," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Kathryn Tunyasuvunakool & Jonas Adler & Zachary Wu & Tim Green & Michal Zielinski & Augustin Žídek & Alex Bridgland & Andrew Cowie & Clemens Meyer & Agata Laydon & Sameer Velankar & Gerard J. Kleywegt, 2021. "Highly accurate protein structure prediction for the human proteome," Nature, Nature, vol. 596(7873), pages 590-596, August.
    3. Hannah G. Hampton & Bridget N. J. Watson & Peter C. Fineran, 2020. "The arms race between bacteria and their phage foes," Nature, Nature, vol. 577(7790), pages 327-336, January.
    4. C. K. Stover & X. Q. Pham & A. L. Erwin & S. D. Mizoguchi & P. Warrener & M. J. Hickey & F.S. L. Brinkman & W. O. Hufnagle & D. J. Kowalik & M. Lagrou & R. L. Garber & L. Goltry & E. Tolentino & S. We, 2000. "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen," Nature, Nature, vol. 406(6799), pages 959-964, August.
    5. John Jumper & Richard Evans & Alexander Pritzel & Tim Green & Michael Figurnov & Olaf Ronneberger & Kathryn Tunyasuvunakool & Russ Bates & Augustin Žídek & Anna Potapenko & Alex Bridgland & Clemens Me, 2021. "Highly accurate protein structure prediction with AlphaFold," Nature, Nature, vol. 596(7873), pages 583-589, August.
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