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Green synthesis of stable hybrid biocatalyst using a hydrogen-bonded, π-π-stacking supramolecular assembly for electrochemical immunosensor

Author

Listed:
  • Wei Huang

    (Sun Yat-sen University
    Sun Yat-sen University)

  • Haitao Yuan

    (Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University))

  • Huangsheng Yang

    (Sun Yat-sen University)

  • Xiaomin Ma

    (Southern University of Science and Technology)

  • Shuyao Huang

    (Sun Yat-sen University)

  • Hongjie Zhang

    (Sun Yat-sen University)

  • Siming Huang

    (Guangzhou Medical University)

  • Guosheng Chen

    (Sun Yat-sen University)

  • Gangfeng Ouyang

    (Sun Yat-sen University)

Abstract

Rational integration of native enzymes and nanoscaffold is an efficient means to access robust biocatalyst, yet remains on-going challenges due to the trade-off between fragile enzymes and harsh assembling conditions. Here, we report a supramolecular strategy enabling the in situ fusion of fragile enzymes into a robust porous crystal. A c2-symmetric pyrene tecton with four formic acid arms is utilized as the building block to engineer this hybrid biocatalyst. The decorated formic acid arms afford the pyrene tectons high dispersibility in minute amount of organic solvent, and permit the hydrogen-bonded linkage of discrete pyrene tectons to an extended supramolecular network around an enzyme in almost organic solvent-free aqueous solution. This hybrid biocatalyst is covered by long-range ordered pore channels, which can serve as the gating to sieve the catalytic substrate and thus enhance the biocatalytic selectivity. Given the structural integration, a supramolecular biocatalyst-based electrochemical immunosensor is developed, enabling the pg/mL detection of cancer biomarker.

Suggested Citation

  • Wei Huang & Haitao Yuan & Huangsheng Yang & Xiaomin Ma & Shuyao Huang & Hongjie Zhang & Siming Huang & Guosheng Chen & Gangfeng Ouyang, 2023. "Green synthesis of stable hybrid biocatalyst using a hydrogen-bonded, π-π-stacking supramolecular assembly for electrochemical immunosensor," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39364-x
    DOI: 10.1038/s41467-023-39364-x
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    References listed on IDEAS

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    1. U. T. Bornscheuer & G. W. Huisman & R. J. Kazlauskas & S. Lutz & J. C. Moore & K. Robins, 2012. "Engineering the third wave of biocatalysis," Nature, Nature, vol. 485(7397), pages 185-194, May.
    2. Linjing Tong & Siming Huang & Yujian Shen & Suya Liu & Xiaomin Ma & Fang Zhu & Guosheng Chen & Gangfeng Ouyang, 2022. "Atomically unveiling the structure-activity relationship of biomacromolecule-metal-organic frameworks symbiotic crystal," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    3. Guosheng Chen & Linjing Tong & Siming Huang & Shuyao Huang & Fang Zhu & Gangfeng Ouyang, 2022. "Hydrogen-bonded organic framework biomimetic entrapment allowing non-native biocatalytic activity in enzyme," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    4. Xiaoling Wu & Hua Yue & Yuanyu Zhang & Xiaoyong Gao & Xiaoyang Li & Licheng Wang & Yufei Cao & Miao Hou & Haixia An & Lin Zhang & Sai Li & Jingyuan Ma & He Lin & Yanan Fu & Hongkai Gu & Wenyong Lou & , 2019. "Packaging and delivering enzymes by amorphous metal-organic frameworks," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    5. Kang Liang & Raffaele Ricco & Cara M. Doherty & Mark J. Styles & Stephen Bell & Nigel Kirby & Stephen Mudie & David Haylock & Anita J. Hill & Christian J. Doonan & Paolo Falcaro, 2015. "Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules," Nature Communications, Nature, vol. 6(1), pages 1-8, November.
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