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Hydrogen-bonded organic framework biomimetic entrapment allowing non-native biocatalytic activity in enzyme

Author

Listed:
  • Guosheng Chen

    (Sun Yat-sen University)

  • Linjing Tong

    (Sun Yat-sen University)

  • Siming Huang

    (Guangzhou Medical University)

  • Shuyao Huang

    (Sun Yat-sen University)

  • Fang Zhu

    (Sun Yat-sen University)

  • Gangfeng Ouyang

    (Sun Yat-sen University
    Sun Yat-sen University)

Abstract

Nature programs the structural folding of an enzyme that allows its on-demand biofunctionality; however, it is still a long-standing challenge to manually modulate an enzyme’s conformation. Here, we design an exogenous hydrogen-bonded organic framework to modulate the conformation of cytochrome c, and hence allow non-native bioactivity for the enzyme. The rigid hydrogen-bonded organic framework, with net-arranged carboxylate inner cage, is in situ installed onto the native cytochrome c. The resultant hydrogen-bonded nano-biointerface changes the conformation to a previously not achieved catalase-like species within the reported cytochrome c-porous organic framework systems. In addition, the preserved hydrogen-bonded organic framework can stabilize the encapsulated enzyme and its channel-like pores also guarantee the free entrance of catalytic substrates. This work describes a conceptual nanotechnology for manoeuvring the flexible conformations of an enzyme, and also highlights the advantages of artificial hydrogen-bonded scaffolds to modulate enzyme activity.

Suggested Citation

  • Guosheng Chen & Linjing Tong & Siming Huang & Shuyao Huang & Fang Zhu & Gangfeng Ouyang, 2022. "Hydrogen-bonded organic framework biomimetic entrapment allowing non-native biocatalytic activity in enzyme," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32454-2
    DOI: 10.1038/s41467-022-32454-2
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    References listed on IDEAS

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    1. Linjing Tong & Siming Huang & Yujian Shen & Suya Liu & Xiaomin Ma & Fang Zhu & Guosheng Chen & Gangfeng Ouyang, 2022. "Atomically unveiling the structure-activity relationship of biomacromolecule-metal-organic frameworks symbiotic crystal," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    2. Kang Liang & Raffaele Ricco & Cara M. Doherty & Mark J. Styles & Stephen Bell & Nigel Kirby & Stephen Mudie & David Haylock & Anita J. Hill & Christian J. Doonan & Paolo Falcaro, 2015. "Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules," Nature Communications, Nature, vol. 6(1), pages 1-8, November.
    3. Christopher M. Dobson, 2003. "Protein folding and misfolding," Nature, Nature, vol. 426(6968), pages 884-890, December.
    4. F. Ulrich Hartl & Andreas Bracher & Manajit Hayer-Hartl, 2011. "Molecular chaperones in protein folding and proteostasis," Nature, Nature, vol. 475(7356), pages 324-332, July.
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    Citations

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    Cited by:

    1. Xiaojun Ding & Jing Chen & Gang Ye, 2024. "Supramolecular polynuclear clusters sustained cubic hydrogen bonded frameworks with octahedral cages for reversible photochromism," Nature Communications, Nature, vol. 15(1), pages 1-9, December.
    2. Shimin Chen & Yan Ju & Yisi Yang & Fahui Xiang & Zizhu Yao & Hao Zhang & Yunbin Li & Yongfan Zhang & Shengchang Xiang & Banglin Chen & Zhangjing Zhang, 2024. "Multistate structures in a hydrogen-bonded polycatenation non-covalent organic framework with diverse resistive switching behaviors," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    3. Wei Huang & Haitao Yuan & Huangsheng Yang & Xiaomin Ma & Shuyao Huang & Hongjie Zhang & Siming Huang & Guosheng Chen & Gangfeng Ouyang, 2023. "Green synthesis of stable hybrid biocatalyst using a hydrogen-bonded, π-π-stacking supramolecular assembly for electrochemical immunosensor," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    4. Xiaoting Hao & Shuo Wang & Xiaoming Zhang & Zhiqiang Ma & Ming Zhang & Hu Shi & Hengquan Yang, 2024. "Engineering enzyme conformation within liquid-solid hybrid microreactors for enhanced continuous-flow biocatalysis," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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