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Packaging and delivering enzymes by amorphous metal-organic frameworks

Author

Listed:
  • Xiaoling Wu

    (Tsinghua University
    South China University of Technology)

  • Hua Yue

    (Chinese Academy of Sciences)

  • Yuanyu Zhang

    (Tsinghua University)

  • Xiaoyong Gao

    (Chinese Academy of Sciences)

  • Xiaoyang Li

    (Tsinghua University)

  • Licheng Wang

    (Tsinghua University)

  • Yufei Cao

    (Tsinghua University)

  • Miao Hou

    (Tsinghua University)

  • Haixia An

    (Chinese Academy of Sciences)

  • Lin Zhang

    (Tianjin University)

  • Sai Li

    (Tsinghua University
    Tsinghua-Peking Joint Center for Life Sciences
    Beijing Advanced Innovation Center for Structural Biology)

  • Jingyuan Ma

    (Chinese Academy of Sciences)

  • He Lin

    (Chinese Academy of Sciences)

  • Yanan Fu

    (Chinese Academy of Sciences)

  • Hongkai Gu

    (Jilin University)

  • Wenyong Lou

    (South China University of Technology)

  • Wei Wei

    (Chinese Academy of Sciences)

  • Richard N. Zare

    (Fudan University, Jiangwan Campus)

  • Jun Ge

    (Tsinghua University
    Tsinghua Shenzhen International Graduate School)

Abstract

Enzymatic catalysis in living cells enables the in-situ detection of cellular metabolites in single cells, which could contribute to early diagnosis of diseases. In this study, enzyme is packaged in amorphous metal-organic frameworks (MOFs) via a one-pot co-precipitation process under ambient conditions, exhibiting 5–20 times higher apparent activity than when the enzyme is encapsulated in corresponding crystalline MOFs. Molecular simulation and cryo-electron tomography (Cryo-ET) combined with other techniques demonstrate that the mesopores generated in this disordered and fuzzy structure endow the packaged enzyme with high enzyme activity. The highly active glucose oxidase delivered by the amorphous MOF nanoparticles allows the noninvasive and facile measurement of glucose in single living cells, which can be used to distinguish between cancerous and normal cells.

Suggested Citation

  • Xiaoling Wu & Hua Yue & Yuanyu Zhang & Xiaoyong Gao & Xiaoyang Li & Licheng Wang & Yufei Cao & Miao Hou & Haixia An & Lin Zhang & Sai Li & Jingyuan Ma & He Lin & Yanan Fu & Hongkai Gu & Wenyong Lou & , 2019. "Packaging and delivering enzymes by amorphous metal-organic frameworks," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13153-x
    DOI: 10.1038/s41467-019-13153-x
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    Citations

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    Cited by:

    1. Wei Zhang & Yanchen Liu & Henrik S. Jeppesen & Nicola Pinna, 2024. "Stöber method to amorphous metal-organic frameworks and coordination polymers," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Jiabin Wu & Xianyu Zhu & Qun Li & Qiang Fu & Bingxue Wang & Beibei Li & Shanshan Wang & Qingchao Chang & Huandong Xiang & Chengliang Ye & Qiqiang Li & Liang Huang & Yan Liang & Dingsheng Wang & Yulian, 2024. "Enhancing radiation-resistance and peroxidase-like activity of single-atom copper nanozyme via local coordination manipulation," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Wei Huang & Haitao Yuan & Huangsheng Yang & Xiaomin Ma & Shuyao Huang & Hongjie Zhang & Siming Huang & Guosheng Chen & Gangfeng Ouyang, 2023. "Green synthesis of stable hybrid biocatalyst using a hydrogen-bonded, π-π-stacking supramolecular assembly for electrochemical immunosensor," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    4. Linjing Tong & Siming Huang & Yujian Shen & Suya Liu & Xiaomin Ma & Fang Zhu & Guosheng Chen & Gangfeng Ouyang, 2022. "Atomically unveiling the structure-activity relationship of biomacromolecule-metal-organic frameworks symbiotic crystal," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    5. Sassone, Daniele & Bocchini, Sergio & Fontana, Marco & Salvini, Clara & Cicero, Giancarlo & Re Fiorentin, Michele & Risplendi, Francesca & Latini, Giulio & Amin Farkhondehfal, M. & Pirri, Fabrizio & Z, 2022. "Imidazole-imidazolate pair as organo-electrocatalyst for CO2 reduction on ZIF-8 material," Applied Energy, Elsevier, vol. 324(C).
    6. Ke Li & Yucheng Zhao & Jian Yang & Jinlou Gu, 2022. "Nanoemulsion-directed growth of MOFs with versatile architectures for the heterogeneous regeneration of coenzymes," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    7. Danping Tian & Ruipeng Hao & Xiaoming Zhang & Hu Shi & Yuwei Wang & Linfeng Liang & Haichao Liu & Hengquan Yang, 2023. "Multi-compartmental MOF microreactors derived from Pickering double emulsions for chemo-enzymatic cascade catalysis," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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