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Engineering enzyme conformation within liquid-solid hybrid microreactors for enhanced continuous-flow biocatalysis

Author

Listed:
  • Xiaoting Hao

    (Shanxi University
    Shanxi University
    Henan University)

  • Shuo Wang

    (Shanxi University)

  • Xiaoming Zhang

    (Shanxi University
    Henan University)

  • Zhiqiang Ma

    (Shanxi University)

  • Ming Zhang

    (Shanxi University
    Shanxi University)

  • Hu Shi

    (Shanxi University)

  • Hengquan Yang

    (Shanxi University
    Shanxi University)

Abstract

The artificial engineering of an enzyme’s structural conformation and dynamic properties to promote its catalytic activity and stability outside cellular environments is highly pursued in industrial biotechnology. Here, we describe an elegant strategy of combining the rationally designed liquid-solid hybrid microreactor with a tailor-made polyethylene glycol functional ionic liquid (PEG-IL) microenvironment to exercise a high level of control over the configuration of enzymes for practical continuous-flow biocatalysis. As exemplified by a lipase driven kinetic resolution reaction, the obtained system exhibits a 2.70 to 30.35-fold activity enhancement compared to their batch or traditional IL-based counterparts. Also, our results demonstrate that the thermal stability of encapsulated lipase can be significantly strengthened in the presence of PEG groups, showcasing a long-term continuous-flow stability even up to 1000 h at evaluated temperature of 60 oC. Through systematic experiment and molecular dynamics simulation studies, the conformational changes of the active site cavity in the modified lipases are correlated with enzymatic properties alteration, and the pronounced effects of PEG-groups in stabilizing enzyme’s secondary structures by delaying unfolding at elevated temperatures are identified. We believe that this study will guide the design of high-performance enzymatic systems, promoting their utilization in real-world biocatalysis applications.

Suggested Citation

  • Xiaoting Hao & Shuo Wang & Xiaoming Zhang & Zhiqiang Ma & Ming Zhang & Hu Shi & Hengquan Yang, 2024. "Engineering enzyme conformation within liquid-solid hybrid microreactors for enhanced continuous-flow biocatalysis," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54725-w
    DOI: 10.1038/s41467-024-54725-w
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    References listed on IDEAS

    as
    1. Alexander M. Klibanov, 2001. "Improving enzymes by using them in organic solvents," Nature, Nature, vol. 409(6817), pages 241-246, January.
    2. Guosheng Chen & Linjing Tong & Siming Huang & Shuyao Huang & Fang Zhu & Gangfeng Ouyang, 2022. "Hydrogen-bonded organic framework biomimetic entrapment allowing non-native biocatalytic activity in enzyme," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
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