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Cryogenic electron ptychographic single particle analysis with wide bandwidth information transfer

Author

Listed:
  • Xudong Pei

    (Nanjing University)

  • Liqi Zhou

    (Nanjing University
    University of Warwick)

  • Chen Huang

    (Harwell Science and Innovation Campus)

  • Mark Boyce

    (University of Oxford)

  • Judy S. Kim

    (Harwell Science and Innovation Campus
    University of Oxford)

  • Emanuela Liberti

    (Harwell Science and Innovation Campus)

  • Yiming Hu

    (Nanjing University)

  • Takeo Sasaki

    (JEOL Ltd)

  • Peter D. Nellist

    (University of Oxford)

  • Peijun Zhang

    (University of Oxford
    Harwell Science and Innovation Campus)

  • David I. Stuart

    (University of Oxford
    Harwell Science and Innovation Campus)

  • Angus I. Kirkland

    (Harwell Science and Innovation Campus
    University of Oxford
    Harwell Science and Innovation Campus)

  • Peng Wang

    (Nanjing University
    University of Warwick)

Abstract

Advances in cryogenic transmission electron microscopy have revolutionised the determination of many macromolecular structures at atomic or near-atomic resolution. This method is based on conventional defocused phase contrast imaging. However, it has limitations of weaker contrast for small biological molecules embedded in vitreous ice, in comparison with cryo-ptychography, which shows increased contrast. Here we report a single-particle analysis based on the use of ptychographic reconstruction data, demonstrating that three dimensional reconstructions with a wide information transfer bandwidth can be recovered by Fourier domain synthesis. Our work suggests future applications in otherwise challenging single particle analyses, including small macromolecules and heterogeneous or flexible particles. In addition structure determination in situ within cells without the requirement for protein purification and expression may be possible.

Suggested Citation

  • Xudong Pei & Liqi Zhou & Chen Huang & Mark Boyce & Judy S. Kim & Emanuela Liberti & Yiming Hu & Takeo Sasaki & Peter D. Nellist & Peijun Zhang & David I. Stuart & Angus I. Kirkland & Peng Wang, 2023. "Cryogenic electron ptychographic single particle analysis with wide bandwidth information transfer," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38268-0
    DOI: 10.1038/s41467-023-38268-0
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    References listed on IDEAS

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    3. Si Gao & Peng Wang & Fucai Zhang & Gerardo T. Martinez & Peter D. Nellist & Xiaoqing Pan & Angus I. Kirkland, 2017. "Electron ptychographic microscopy for three-dimensional imaging," Nature Communications, Nature, vol. 8(1), pages 1-8, December.
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    Cited by:

    1. Berk Küçükoğlu & Inayathulla Mohammed & Ricardo C. Guerrero-Ferreira & Stephanie M. Ribet & Georgios Varnavides & Max Leo Leidl & Kelvin Lau & Sergey Nazarov & Alexander Myasnikov & Massimo Kube & Jul, 2024. "Low-dose cryo-electron ptychography of proteins at sub-nanometer resolution," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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