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Atomic-level structural responsiveness to environmental conditions from 3D electron diffraction

Author

Listed:
  • Yang Ling

    (ShanghaiTech University)

  • Tu Sun

    (ShanghaiTech University)

  • Linshuo Guo

    (ShanghaiTech University)

  • Xiaomeng Si

    (ShanghaiTech University)

  • Yilan Jiang

    (ShanghaiTech University
    ShanghaiTech University)

  • Qing Zhang

    (ShanghaiTech University
    ShanghaiTech University)

  • Zhaoxi Chen

    (ShanghaiTech University)

  • Osamu Terasaki

    (ShanghaiTech University
    ShanghaiTech University)

  • Yanhang Ma

    (ShanghaiTech University
    ShanghaiTech University)

Abstract

Electron microscopy has been widely used in the structural analysis of proteins, pharmaceutical products, and various functional materials in the past decades. However, one fact is often overlooked that the crystal structure might be sensitive to external environments and response manners, which will bring uncertainty to the structure determination and structure-property correlation. Here, we report the atomic-level ab initio structure determinations of microcrystals by combining 3D electron diffraction (3D ED) and environmental transmission electron microscope (TEM). Environmental conditions, including cryo, heating, gas and liquid, have been successfully achieved using in situ holders to reveal the simuli-responsive structures of crystals. Remarkable structural changes have been directly resolved by 3D ED in one flexible metal-organic framework, MIL-53, owing to the response of framework to pressures, temperatures, guest molecules, etc.

Suggested Citation

  • Yang Ling & Tu Sun & Linshuo Guo & Xiaomeng Si & Yilan Jiang & Qing Zhang & Zhaoxi Chen & Osamu Terasaki & Yanhang Ma, 2022. "Atomic-level structural responsiveness to environmental conditions from 3D electron diffraction," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34237-1
    DOI: 10.1038/s41467-022-34237-1
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    References listed on IDEAS

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    1. Ka Man Yip & Niels Fischer & Elham Paknia & Ashwin Chari & Holger Stark, 2020. "Atomic-resolution protein structure determination by cryo-EM," Nature, Nature, vol. 587(7832), pages 157-161, November.
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