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The translating bacterial ribosome at 1.55 Å resolution generated by cryo-EM imaging services

Author

Listed:
  • Simon A. Fromm

    (European Molecular Biology Laboratory)

  • Kate M. O’Connor

    (University College Cork)

  • Michael Purdy

    (University of Virginia)

  • Pramod R. Bhatt

    (University College Cork)

  • Gary Loughran

    (University College Cork)

  • John F. Atkins

    (University College Cork
    MRC Laboratory of Molecular Biology)

  • Ahmad Jomaa

    (University of Virginia
    University of Virginia)

  • Simone Mattei

    (European Molecular Biology Laboratory
    European Molecular Biology Laboratory)

Abstract

Our understanding of protein synthesis has been conceptualised around the structure and function of the bacterial ribosome. This complex macromolecular machine is the target of important antimicrobial drugs, an integral line of defence against infectious diseases. Here, we describe how open access to cryo-electron microscopy facilities combined with bespoke user support enabled structural determination of the translating ribosome from Escherichia coli at 1.55 Å resolution. The obtained structures allow for direct determination of the rRNA sequence to identify ribosome polymorphism sites in the E. coli strain used in this study and enable interpretation of the ribosomal active and peripheral sites at unprecedented resolution. This includes scarcely populated chimeric hybrid states of the ribosome engaged in several tRNA translocation steps resolved at ~2 Å resolution. The current map not only improves our understanding of protein synthesis but also allows for more precise structure-based drug design of antibiotics to tackle rising bacterial resistance.

Suggested Citation

  • Simon A. Fromm & Kate M. O’Connor & Michael Purdy & Pramod R. Bhatt & Gary Loughran & John F. Atkins & Ahmad Jomaa & Simone Mattei, 2023. "The translating bacterial ribosome at 1.55 Å resolution generated by cryo-EM imaging services," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36742-3
    DOI: 10.1038/s41467-023-36742-3
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    1. Pavel V. Panteleev & Eugene B. Pichkur & Roman N. Kruglikov & Alena Paleskava & Olga V. Shulenina & Ilia A. Bolosov & Ivan V. Bogdanov & Victoria N. Safronova & Sergey V. Balandin & Valeriya I. Marina, 2024. "Rumicidins are a family of mammalian host-defense peptides plugging the 70S ribosome exit tunnel," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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