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Three-dimensional electron ptychography of organic–inorganic hybrid nanostructures

Author

Listed:
  • Zhiyuan Ding

    (Nanjing University
    University of Oxford)

  • Si Gao

    (Nanjing University
    Nanjing Tech University)

  • Weina Fang

    (East China Normal University)

  • Chen Huang

    (University of Oxford
    Harwell Campus)

  • Liqi Zhou

    (Nanjing University)

  • Xudong Pei

    (Nanjing University)

  • Xiaoguo Liu

    (Shanghai Jiao Tong University)

  • Xiaoqing Pan

    (University of California)

  • Chunhai Fan

    (Shanghai Jiao Tong University)

  • Angus I. Kirkland

    (University of Oxford
    Harwell Campus
    Harwell Science and Innovation Campus)

  • Peng Wang

    (Nanjing University
    University of Warwick)

Abstract

Three dimensional scaffolded DNA origami with inorganic nanoparticles has been used to create tailored multidimensional nanostructures. However, the image contrast of DNA is poorer than those of the heavy nanoparticles in conventional transmission electron microscopy at high defocus so that the biological and non-biological components in 3D scaffolds cannot be simultaneously resolved using tomography of samples in a native state. We demonstrate the use of electron ptychography to recover high contrast phase information from all components in a DNA origami scaffold without staining. We further quantitatively evaluate the enhancement of contrast in comparison with conventional transmission electron microscopy. In addition, We show that for ptychography post-reconstruction focusing simplifies the workflow and reduces electron dose and beam damage.

Suggested Citation

  • Zhiyuan Ding & Si Gao & Weina Fang & Chen Huang & Liqi Zhou & Xudong Pei & Xiaoguo Liu & Xiaoqing Pan & Chunhai Fan & Angus I. Kirkland & Peng Wang, 2022. "Three-dimensional electron ptychography of organic–inorganic hybrid nanostructures," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32548-x
    DOI: 10.1038/s41467-022-32548-x
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    as
    1. Anton Kuzyk & Robert Schreiber & Zhiyuan Fan & Günther Pardatscher & Eva-Maria Roller & Alexander Högele & Friedrich C. Simmel & Alexander O. Govorov & Tim Liedl, 2012. "DNA-based self-assembly of chiral plasmonic nanostructures with tailored optical response," Nature, Nature, vol. 483(7389), pages 311-314, March.
    2. Jianwei Miao & Pambos Charalambous & Janos Kirz & David Sayre, 1999. "Extending the methodology of X-ray crystallography to allow imaging of micrometre-sized non-crystalline specimens," Nature, Nature, vol. 400(6742), pages 342-344, July.
    3. H. Yang & R. N. Rutte & L. Jones & M. Simson & R. Sagawa & H. Ryll & M. Huth & T. J. Pennycook & M.L.H. Green & H. Soltau & Y. Kondo & B. G. Davis & P. D. Nellist, 2016. "Simultaneous atomic-resolution electron ptychography and Z-contrast imaging of light and heavy elements in complex nanostructures," Nature Communications, Nature, vol. 7(1), pages 1-8, November.
    4. Martin Dierolf & Andreas Menzel & Pierre Thibault & Philipp Schneider & Cameron M. Kewish & Roger Wepf & Oliver Bunk & Franz Pfeiffer, 2010. "Ptychographic X-ray computed tomography at the nanoscale," Nature, Nature, vol. 467(7314), pages 436-439, September.
    5. Si Gao & Peng Wang & Fucai Zhang & Gerardo T. Martinez & Peter D. Nellist & Xiaoqing Pan & Angus I. Kirkland, 2017. "Electron ptychographic microscopy for three-dimensional imaging," Nature Communications, Nature, vol. 8(1), pages 1-8, December.
    6. Huajie Liu & Jianbang Wang & Shiping Song & Chunhai Fan & Kurt V. Gothelf, 2015. "A DNA-based system for selecting and displaying the combined result of two input variables," Nature Communications, Nature, vol. 6(1), pages 1-7, December.
    7. Yi Jiang & Zhen Chen & Yimo Han & Pratiti Deb & Hui Gao & Saien Xie & Prafull Purohit & Mark W. Tate & Jiwoong Park & Sol M. Gruner & Veit Elser & David A. Muller, 2018. "Electron ptychography of 2D materials to deep sub-ångström resolution," Nature, Nature, vol. 559(7714), pages 343-349, July.
    8. Liqi Zhou & Jingdong Song & Judy S. Kim & Xudong Pei & Chen Huang & Mark Boyce & Luiza Mendonça & Daniel Clare & Alistair Siebert & Christopher S. Allen & Emanuela Liberti & David Stuart & Xiaoqing Pa, 2020. "Low-dose phase retrieval of biological specimens using cryo-electron ptychography," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    9. Dongsheng Lei & Alexander E. Marras & Jianfang Liu & Chao-Min Huang & Lifeng Zhou & Carlos E. Castro & Hai-Jun Su & Gang Ren, 2018. "Three-dimensional structural dynamics of DNA origami Bennett linkages using individual-particle electron tomography," Nature Communications, Nature, vol. 9(1), pages 1-8, December.
    10. Maryam Khoshouei & Mazdak Radjainia & Wolfgang Baumeister & Radostin Danev, 2017. "Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate," Nature Communications, Nature, vol. 8(1), pages 1-6, December.
    11. Colin Ophus & Jim Ciston & Jordan Pierce & Tyler R. Harvey & Jordan Chess & Benjamin J. McMorran & Cory Czarnik & Harald H. Rose & Peter Ercius, 2016. "Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry," Nature Communications, Nature, vol. 7(1), pages 1-7, April.
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    1. Philipp M. Pelz & Sinéad M. Griffin & Scott Stonemeyer & Derek Popple & Hannah DeVyldere & Peter Ercius & Alex Zettl & Mary C. Scott & Colin Ophus, 2023. "Solving complex nanostructures with ptychographic atomic electron tomography," Nature Communications, Nature, vol. 14(1), pages 1-9, December.

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