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Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin

Author

Listed:
  • Yongwoo Kwon

    (Institute for Basic Science
    Korea University)

  • Jin Hee Hong

    (Institute for Basic Science
    Korea University)

  • Sungsam Kang

    (Institute for Basic Science
    Korea University)

  • Hojun Lee

    (Institute for Basic Science
    Korea University)

  • Yonghyeon Jo

    (Institute for Basic Science
    Korea University)

  • Ki Hean Kim

    (Pohang University of Science and Technology)

  • Seokchan Yoon

    (Institute for Basic Science
    Korea University
    Pusan National University)

  • Wonshik Choi

    (Institute for Basic Science
    Korea University)

Abstract

Myelination processes are closely related to higher brain functions such as learning and memory. While their longitudinal observation has been crucial to understanding myelin-related physiology and various brain disorders, skull opening or thinning has been required to secure clear optical access. Here we present a high-speed reflection matrix microscope using a light source with a wavelength of 1.3 μm to reduce tissue scattering and aberration. Furthermore, we develop a computational conjugate adaptive optics algorithm designed for the recorded reflection matrix to optimally compensate for the skull aberrations. These developments allow us to realize label-free longitudinal imaging of cortical myelin through an intact mouse skull. The myelination processes of the same mice were observed from 3 to 10 postnatal weeks to the depth of cortical layer 4 with a spatial resolution of 0.79 μm. Our system will expedite the investigations on the role of myelination in learning, memory, and brain disorders.

Suggested Citation

  • Yongwoo Kwon & Jin Hee Hong & Sungsam Kang & Hojun Lee & Yonghyeon Jo & Ki Hean Kim & Seokchan Yoon & Wonshik Choi, 2023. "Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-022-35738-9
    DOI: 10.1038/s41467-022-35738-9
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    References listed on IDEAS

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    1. Moonseok Kim & Yonghyeon Jo & Jin Hee Hong & Suhyun Kim & Seokchan Yoon & Kyung-Deok Song & Sungsam Kang & Byunghak Lee & Guang Hoon Kim & Hae-Chul Park & Wonshik Choi, 2019. "Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    2. Sungsam Kang & Pilsung Kang & Seungwon Jeong & Yongwoo Kwon & Taeseok D. Yang & Jin Hee Hong & Moonseok Kim & Kyung–Deok Song & Jin Hyoung Park & Jun Ho Lee & Myoung Joon Kim & Ki Hean Kim & Wonshik C, 2017. "High-resolution adaptive optical imaging within thick scattering media using closed-loop accumulation of single scattering," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
    3. Klaus-Armin Nave, 2010. "Myelination and support of axonal integrity by glia," Nature, Nature, vol. 468(7321), pages 244-252, November.
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    1. Ulysse Najar & Victor Barolle & Paul Balondrade & Mathias Fink & Claude Boccara & Alexandre Aubry, 2024. "Harnessing forward multiple scattering for optical imaging deep inside an opaque medium," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    2. Flavien Bureau & Justine Robin & Arthur Ber & William Lambert & Mathias Fink & Alexandre Aubry, 2023. "Three-dimensional ultrasound matrix imaging," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. Sanghyeon Park & Yonghyeon Jo & Minsu Kang & Jin Hee Hong & Sangyoon Ko & Suhyun Kim & Sangjun Park & Hae Chul Park & Sang-Hee Shim & Wonshik Choi, 2023. "Label-free adaptive optics single-molecule localization microscopy for whole zebrafish," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

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