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Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm

Author

Listed:
  • Moonseok Kim

    (Institute for Basic Science
    Korea University
    The Catholic University of Korea
    The Catholic University of Korea)

  • Yonghyeon Jo

    (Institute for Basic Science
    Korea University)

  • Jin Hee Hong

    (Institute for Basic Science
    Korea University)

  • Suhyun Kim

    (Korea University)

  • Seokchan Yoon

    (Institute for Basic Science
    Korea University)

  • Kyung-Deok Song

    (Institute for Basic Science
    Korea University)

  • Sungsam Kang

    (Massachusetts Institute of Technology)

  • Byunghak Lee

    (Korea Electrotechnology Research Institute)

  • Guang Hoon Kim

    (Korea Electrotechnology Research Institute)

  • Hae-Chul Park

    (Korea University)

  • Wonshik Choi

    (Institute for Basic Science
    Korea University)

Abstract

Label-free in vivo imaging is crucial for elucidating the underlying mechanisms of many important biological systems in their most native states. However, the applicability of existing modalities has been limited to either superficial layers or early developmental stages due to tissue turbidity. Here, we report a synchronous angular scanning microscope for the rapid interferometric recording of the time-gated reflection matrix, which is a unique matrix characterizing full light-specimen interaction. By applying single scattering accumulation algorithm to the recorded matrix, we removed both high-order sample-induced aberrations and multiple scattering noise with the effective aberration correction speed of 10,000 modes/s. We demonstrated in vivo imaging of whole neural network throughout the hindbrain of the larval zebrafish at a matured stage where physical dissection used to be required for conventional imaging. Our method will expand the scope of applications for optical imaging, where fully non-invasive interrogation of living specimens is critical.

Suggested Citation

  • Moonseok Kim & Yonghyeon Jo & Jin Hee Hong & Suhyun Kim & Seokchan Yoon & Kyung-Deok Song & Sungsam Kang & Byunghak Lee & Guang Hoon Kim & Hae-Chul Park & Wonshik Choi, 2019. "Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11040-z
    DOI: 10.1038/s41467-019-11040-z
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    Cited by:

    1. Sanghyeon Park & Yonghyeon Jo & Minsu Kang & Jin Hee Hong & Sangyoon Ko & Suhyun Kim & Sangjun Park & Hae Chul Park & Sang-Hee Shim & Wonshik Choi, 2023. "Label-free adaptive optics single-molecule localization microscopy for whole zebrafish," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Yongwoo Kwon & Jin Hee Hong & Sungsam Kang & Hojun Lee & Yonghyeon Jo & Ki Hean Kim & Seokchan Yoon & Wonshik Choi, 2023. "Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    3. Wonjun Choi & Munkyu Kang & Jin Hee Hong & Ori Katz & Byunghak Lee & Guang Hoon Kim & Youngwoon Choi & Wonshik Choi, 2022. "Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    4. Ye-Ryoung Lee & Dong-Young Kim & Yonghyeon Jo & Moonseok Kim & Wonshik Choi, 2023. "Exploiting volumetric wave correlation for enhanced depth imaging in scattering medium," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

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