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Label-free adaptive optics single-molecule localization microscopy for whole zebrafish

Author

Listed:
  • Sanghyeon Park

    (Institute for Basic Science
    Korea University)

  • Yonghyeon Jo

    (Institute for Basic Science
    Korea University)

  • Minsu Kang

    (Korea University)

  • Jin Hee Hong

    (Institute for Basic Science)

  • Sangyoon Ko

    (Korea University)

  • Suhyun Kim

    (Korea University)

  • Sangjun Park

    (The Catholic University of Korea
    The Catholic University of Korea)

  • Hae Chul Park

    (Korea University)

  • Sang-Hee Shim

    (Korea University)

  • Wonshik Choi

    (Institute for Basic Science
    Korea University)

Abstract

Specimen-induced aberration has been a major factor limiting the imaging depth of single-molecule localization microscopy (SMLM). Here, we report the application of label-free wavefront sensing adaptive optics to SMLM for deep-tissue super-resolution imaging. The proposed system measures complex tissue aberrations from intrinsic reflectance rather than fluorescence emission and physically corrects the wavefront distortion more than three-fold stronger than the previous limit. This enables us to resolve sub-diffraction morphologies of cilia and oligodendrocytes in whole zebrafish as well as dendritic spines in thick mouse brain tissues at the depth of up to 102 μm with localization number enhancement by up to 37 times and localization precision comparable to aberration-free samples. The proposed approach can expand the application range of SMLM to whole zebrafish that cause the loss of localization number owing to severe tissue aberrations.

Suggested Citation

  • Sanghyeon Park & Yonghyeon Jo & Minsu Kang & Jin Hee Hong & Sangyoon Ko & Suhyun Kim & Sangjun Park & Hae Chul Park & Sang-Hee Shim & Wonshik Choi, 2023. "Label-free adaptive optics single-molecule localization microscopy for whole zebrafish," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39896-2
    DOI: 10.1038/s41467-023-39896-2
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    References listed on IDEAS

    as
    1. Marijn E. Siemons & Naomi A. K. Hanemaaijer & Maarten H. P. Kole & Lukas C. Kapitein, 2021. "Robust adaptive optics for localization microscopy deep in complex tissue," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    2. Yongwoo Kwon & Jin Hee Hong & Sungsam Kang & Hojun Lee & Yonghyeon Jo & Ki Hean Kim & Seokchan Yoon & Wonshik Choi, 2023. "Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    3. Moonseok Kim & Yonghyeon Jo & Jin Hee Hong & Suhyun Kim & Seokchan Yoon & Kyung-Deok Song & Sungsam Kang & Byunghak Lee & Guang Hoon Kim & Hae-Chul Park & Wonshik Choi, 2019. "Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    4. Seokchan Yoon & Hojun Lee & Jin Hee Hong & Yong-Sik Lim & Wonshik Choi, 2020. "Laser scanning reflection-matrix microscopy for aberration-free imaging through intact mouse skull," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    5. Sungsam Kang & Pilsung Kang & Seungwon Jeong & Yongwoo Kwon & Taeseok D. Yang & Jin Hee Hong & Moonseok Kim & Kyung–Deok Song & Jin Hyoung Park & Jun Ho Lee & Myoung Joon Kim & Ki Hean Kim & Wonshik C, 2017. "High-resolution adaptive optical imaging within thick scattering media using closed-loop accumulation of single scattering," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
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