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Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues

Author

Listed:
  • Wonjun Choi

    (Institute for Basic Science
    Korea University)

  • Munkyu Kang

    (Institute for Basic Science
    Korea University)

  • Jin Hee Hong

    (Institute for Basic Science
    Korea University)

  • Ori Katz

    (The Hebrew University of Jerusalem)

  • Byunghak Lee

    (Korea Electrotechnology Research Institute
    B2LAB co., ltd)

  • Guang Hoon Kim

    (Korea Electrotechnology Research Institute)

  • Youngwoon Choi

    (Korea University
    Korea University)

  • Wonshik Choi

    (Institute for Basic Science
    Korea University)

Abstract

Ultrathin lensless fibre endoscopes offer minimally invasive investigation, but they mostly operate as a rigid type due to the need for prior calibration of a fibre probe. Furthermore, most implementations work in fluorescence mode rather than label-free imaging mode, making them unsuitable for general medical diagnosis. Herein, we report a fully flexible ultrathin fibre endoscope taking 3D holographic images of unstained tissues with 0.85-μm spatial resolution. Using a bare fibre bundle as thin as 200-μm diameter, we design a lensless Fourier holographic imaging configuration to selectively detect weak reflections from biological tissues, a critical step for label-free endoscopic reflectance imaging. A unique algorithm is developed for calibration-free holographic image reconstruction, allowing us to image through a narrow and curved passage regardless of fibre bending. We demonstrate endoscopic reflectance imaging of unstained rat intestine tissues that are completely invisible to conventional endoscopes. The proposed endoscope will expedite a more accurate and earlier diagnosis than before with minimal complications.

Suggested Citation

  • Wonjun Choi & Munkyu Kang & Jin Hee Hong & Ori Katz & Byunghak Lee & Guang Hoon Kim & Youngwoon Choi & Wonshik Choi, 2022. "Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32114-5
    DOI: 10.1038/s41467-022-32114-5
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    References listed on IDEAS

    as
    1. Tomáš Čižmár & Kishan Dholakia, 2012. "Exploiting multimode waveguides for pure fibre-based imaging," Nature Communications, Nature, vol. 3(1), pages 1-9, January.
    2. Moonseok Kim & Yonghyeon Jo & Jin Hee Hong & Suhyun Kim & Seokchan Yoon & Kyung-Deok Song & Sungsam Kang & Byunghak Lee & Guang Hoon Kim & Hae-Chul Park & Wonshik Choi, 2019. "Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    3. Sungsam Kang & Pilsung Kang & Seungwon Jeong & Yongwoo Kwon & Taeseok D. Yang & Jin Hee Hong & Moonseok Kim & Kyung–Deok Song & Jin Hyoung Park & Jun Ho Lee & Myoung Joon Kim & Ki Hean Kim & Wonshik C, 2017. "High-resolution adaptive optical imaging within thick scattering media using closed-loop accumulation of single scattering," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
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