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Single-cell sequencing shows cellular heterogeneity of cutaneous lesions in lupus erythematosus

Author

Listed:
  • Meiling Zheng

    (Central South University
    Chinese Academy of Medical Sciences)

  • Zhi Hu

    (Central South University
    Chinese Academy of Medical Sciences)

  • Xiaole Mei

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Lianlian Ouyang

    (Central South University
    Chinese Academy of Medical Sciences)

  • Yang Song

    (Central South University
    Chinese Academy of Medical Sciences)

  • Wenhui Zhou

    (Central South University
    Chinese Academy of Medical Sciences)

  • Yi Kong

    (Central South University
    Chinese Academy of Medical Sciences)

  • Ruifang Wu

    (Central South University
    Chinese Academy of Medical Sciences)

  • Shijia Rao

    (Central South University
    Chinese Academy of Medical Sciences)

  • Hai Long

    (Central South University
    Chinese Academy of Medical Sciences)

  • Wei Shi

    (Central South University)

  • Hui Jing

    (Central South University
    Chinese Academy of Medical Sciences)

  • Shuang Lu

    (Central South University
    Chinese Academy of Medical Sciences)

  • Haijing Wu

    (Central South University
    Chinese Academy of Medical Sciences)

  • Sujie Jia

    (Central South University)

  • Qianjin Lu

    (Central South University
    Chinese Academy of Medical Sciences
    Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Ming Zhao

    (Central South University
    Chinese Academy of Medical Sciences)

Abstract

Discoid lupus erythematosus (DLE) and systemic lupus erythematosus (SLE) are both types of lupus, yet the characteristics, and differences between them are not fully understood. Here we show single-cell RNA sequencing data of cutaneous lesions from DLE and SLE patients and skin tissues from healthy controls (HCs). We find significantly higher proportions of T cells, B cells and NK cells in DLE than in SLE. Expanded CCL20+ keratinocyte, CXCL1+ fibroblast, ISGhiCD4/CD8 T cell, ISGhi plasma cell, pDC, and NK subclusters are identified in DLE and SLE compared to HC. In addition, we observe higher cell communication scores between cell types such as fibroblasts and macrophage/dendritic cells in cutaneous lesions of DLE and SLE compared to HC. In summary, we clarify the heterogeneous characteristics in cutaneous lesions between DLE and SLE, and discover some specific cell subtypes and ligand-receptor pairs that indicate possible therapeutic targets of lupus erythematosus.

Suggested Citation

  • Meiling Zheng & Zhi Hu & Xiaole Mei & Lianlian Ouyang & Yang Song & Wenhui Zhou & Yi Kong & Ruifang Wu & Shijia Rao & Hai Long & Wei Shi & Hui Jing & Shuang Lu & Haijing Wu & Sujie Jia & Qianjin Lu & , 2022. "Single-cell sequencing shows cellular heterogeneity of cutaneous lesions in lupus erythematosus," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35209-1
    DOI: 10.1038/s41467-022-35209-1
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