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Single-cell transcriptome atlas of the human corpus cavernosum

Author

Listed:
  • LiangYu Zhao

    (Shanghai Jiao Tong University School of Medicine
    Sun Yat-sen University)

  • Sha Han

    (Shanghai Jiao Tong University School of Medicine)

  • HengChuan Su

    (Fudan University)

  • JianYing Li

    (Shanghai Jiao Tong University School of Medicine)

  • ErLei Zhi

    (Shanghai Jiao Tong University School of Medicine)

  • Peng Li

    (Shanghai Jiao Tong University School of Medicine)

  • ChenCheng Yao

    (Shanghai Jiao Tong University School of Medicine)

  • RuHui Tian

    (Shanghai Jiao Tong University School of Medicine)

  • HuiXing Chen

    (Shanghai Jiao Tong University School of Medicine)

  • HuiRong Chen

    (Shanghai Jiao Tong University School of Medicine)

  • JiaQiang Luo

    (Shanghai Jiao Tong University School of Medicine)

  • ChenKun Shi

    (Shanghai Jiao Tong University School of Medicine)

  • ZhiYong Ji

    (Shanghai Jiao Tong University School of Medicine)

  • JianLin Hu

    (Shanghai Jiao Tong University School of Medicine)

  • Gang Wu

    (Tongji University)

  • WeiDong Zhou

    (Tongji University)

  • YuXin Tang

    (Sun Yat-sen University)

  • YuZhuo Chen

    (Sun Yat-sen University)

  • GuiTing Lin

    (University of California)

  • Tom F. Lue

    (University of California)

  • DengLong Wu

    (Fudan University)

  • Zheng Li

    (Shanghai Jiao Tong University School of Medicine)

Abstract

The corpus cavernosum is the most important structure for penile erection, and its dysfunction causes many physiological and psychological problems. However, its cellular heterogeneity and signalling networks at the molecular level are poorly understood because of limited access to samples. Here, we profile 64,993 human cavernosal single-cell transcriptomes from three males with normal erection and five organic erectile dysfunction patients. Cell communication analysis reveals that cavernosal fibroblasts are central to the paracrine signalling network and regulate microenvironmental homeostasis. Combining with immunohistochemical staining, we reveal the cellular heterogeneity and describe a detailed spatial distribution map for each fibroblast, smooth muscle and endothelial subcluster in the corpus cavernosum. Furthermore, comparative analysis and related functional experiments identify candidate regulatory signalling pathways in the pathological process. Our study provides an insight into the human corpus cavernosum microenvironment and a reference for potential erectile dysfunction therapies.

Suggested Citation

  • LiangYu Zhao & Sha Han & HengChuan Su & JianYing Li & ErLei Zhi & Peng Li & ChenCheng Yao & RuHui Tian & HuiXing Chen & HuiRong Chen & JiaQiang Luo & ChenKun Shi & ZhiYong Ji & JianLin Hu & Gang Wu & , 2022. "Single-cell transcriptome atlas of the human corpus cavernosum," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31950-9
    DOI: 10.1038/s41467-022-31950-9
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    References listed on IDEAS

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    1. Mintao Ji & Dongsheng Chen & Yinyin Shu & Shuai Dong & Zhisen Zhang & Haimeng Zheng & Xiaoni Jin & Lijun Zheng & Yang Liu & Yifei Zheng & Wensheng Zhang & Shiyou Wang & Guangming Zhou & Bingyan Li & B, 2023. "The role of mechano-regulated YAP/TAZ in erectile dysfunction," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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