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A single-cell transcriptional atlas reveals resident progenitor cell niche functions in TMJ disc development and injury

Author

Listed:
  • Ruiye Bi

    (Sichuan University)

  • Qing Yin

    (Sichuan University
    W. G. Kerckhoff Institute)

  • Haohan Li

    (Sichuan University)

  • Xianni Yang

    (Sichuan University)

  • Yiru Wang

    (Sichuan University)

  • Qianli Li

    (Sichuan University)

  • Han Fang

    (Sichuan University)

  • Peiran Li

    (Sichuan University)

  • Ping Lyu

    (Sichuan University)

  • Yi Fan

    (Sichuan University)

  • Binbin Ying

    (Ningbo First Hospital)

  • Songsong Zhu

    (Sichuan University)

Abstract

The biological characteristics of the temporomandibular joint disc involve complex cellular network in cell identity and extracellular matrix composition to modulate jaw function. The lack of a detailed characterization of the network severely limits the development of targeted therapies for temporomandibular joint-related diseases. Here we profiled single-cell transcriptomes of disc cells from mice at different postnatal stages, finding that the fibroblast population could be divided into chondrogenic and non-chondrogenic clusters. We also find that the resident mural cell population is the source of disc progenitors, characterized by ubiquitously active expression of the NOTCH3 and THY1 pathways. Lineage tracing reveals that Myh11+ mural cells coordinate angiogenesis during disc injury but lost their progenitor characteristics and ultimately become Sfrp2+ non-chondrogenic fibroblasts instead of Chad+ chondrogenic fibroblasts. Overall, we reveal multiple insights into the coordinated development of disc cells and are the first to describe the resident mural cell progenitor during disc injury.

Suggested Citation

  • Ruiye Bi & Qing Yin & Haohan Li & Xianni Yang & Yiru Wang & Qianli Li & Han Fang & Peiran Li & Ping Lyu & Yi Fan & Binbin Ying & Songsong Zhu, 2023. "A single-cell transcriptional atlas reveals resident progenitor cell niche functions in TMJ disc development and injury," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36406-2
    DOI: 10.1038/s41467-023-36406-2
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