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Oligomerization of Peptides LVEALYL and RGFFYT and Their Binding Affinity to Insulin

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  • Hsin-Lin Chiang
  • Son Tung Ngo
  • Chun-Jung Chen
  • Chin-Kun Hu
  • Mai Suan Li

Abstract

Recently it has been proposed a model for fibrils of human insulin in which the fibril growth proceeds via stacking LVEALYL (fragment 11–17 from chain B of insulin) into pairs of tightly interdigitated -sheets. The experiments have also shown that LVEALYL has high propensity to self-assembly and binding to insulin. This necessitates study of oligomerization of LVEALYL and its binding affinity to full-length insulin. Using the all-atom simulations with Gromos96 43a1 force field and explicit water it is shown that LVEALYL can aggregate. Theoretical estimation of the binding free energy of LVEALYL to insulin by the molecular mechanic Poisson-Boltzmann surface area method reveals its strong binding affinity to chain B, implying that, in agreement with the experiments, LVEALYL can affect insulin aggregation via binding mechanism. We predict that, similar to LVEALYL, peptide RGFFYT (fragment B22-27) can self-assemble and bind to insulin modulating its fibril growth process. The binding affinity of RGFFYT is shown to be comparable with that of LVEALYL.

Suggested Citation

  • Hsin-Lin Chiang & Son Tung Ngo & Chun-Jung Chen & Chin-Kun Hu & Mai Suan Li, 2013. "Oligomerization of Peptides LVEALYL and RGFFYT and Their Binding Affinity to Insulin," PLOS ONE, Public Library of Science, vol. 8(6), pages 1-11, June.
  • Handle: RePEc:plo:pone00:0065358
    DOI: 10.1371/journal.pone.0065358
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    1. Bente Vestergaard & Minna Groenning & Manfred Roessle & Jette S Kastrup & Marco van de Weert & James M Flink & Sven Frokjaer & Michael Gajhede & Dmitri I Svergun, 2007. "A Helical Structural Nucleus Is the Primary Elongating Unit of Insulin Amyloid Fibrils," PLOS Biology, Public Library of Science, vol. 5(5), pages 1-9, May.
    2. Christopher M. Dobson, 2003. "Protein folding and misfolding," Nature, Nature, vol. 426(6968), pages 884-890, December.
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