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RNA-binding protein RBM3 intrinsically suppresses lung innate lymphoid cell activation and inflammation partially through CysLT1R

Author

Listed:
  • Jana H. Badrani

    (University of California San Diego)

  • Allyssa N. Strohm

    (University of California San Diego
    Veterans Affairs San Diego Health Care System)

  • Lee Lacasa

    (University of California San Diego)

  • Blake Civello

    (University of California San Diego)

  • Kellen Cavagnero

    (University of California San Diego)

  • Yung-An Haung

    (University of California San Diego
    Chang Gung University)

  • Michael Amadeo

    (University of California San Diego)

  • Luay H. Naji

    (University of California San Diego)

  • Sean J. Lund

    (University of California San Diego)

  • Anthea Leng

    (University of California San Diego)

  • Hyojoung Kim

    (University of California San Diego)

  • Rachel E. Baum

    (University of California San Diego)

  • Naseem Khorram

    (University of California San Diego)

  • Monalisa Mondal

    (La Jolla Institute)

  • Grégory Seumois

    (La Jolla Institute)

  • Julie Pilotte

    (The Scripps Research Institute)

  • Peter W. Vanderklish

    (The Scripps Research Institute)

  • Heather M. McGee

    (University of California San Diego
    Salk Institute
    City of Hope
    Department of Molecular Medicine)

  • Taylor A. Doherty

    (University of California San Diego
    Veterans Affairs San Diego Health Care System)

Abstract

Innate lymphoid cells (ILC) promote lung inflammation in asthma through cytokine production. RNA-binding proteins (RBPs) are critical post-transcriptional regulators, although less is known about RBPs in ILC biology. Here, we demonstrate that RNA-binding motif 3 (RBM3) is highly expressed in lung ILCs and is further induced by alarmins TSLP and IL-33. Rbm3−/− and Rbm3−/−Rag2−/− mice exposed to asthma-associated Alternaria allergen develop enhanced eosinophilic lung inflammation and ILC activation. IL-33 stimulation studies in vivo and in vitro show that RBM3 suppressed lung ILC responses. Further, Rbm3−/− ILCs from bone marrow chimeric mice display increased ILC cytokine production suggesting an ILC-intrinsic suppressive function of RBM3. RNA-sequencing of Rbm3−/− lung ILCs demonstrates increased expression of type 2/17 cytokines and cysteinyl leukotriene 1 receptor (CysLT1R). Finally, Rbm3−/−Cyslt1r−/− mice show dependence on CysLT1R for accumulation of ST2+IL-17+ ILCs. Thus, RBM3 intrinsically regulates lung ILCs during allergen-induced type 2 inflammation that is partially dependent on CysLT1R.

Suggested Citation

  • Jana H. Badrani & Allyssa N. Strohm & Lee Lacasa & Blake Civello & Kellen Cavagnero & Yung-An Haung & Michael Amadeo & Luay H. Naji & Sean J. Lund & Anthea Leng & Hyojoung Kim & Rachel E. Baum & Nasee, 2022. "RNA-binding protein RBM3 intrinsically suppresses lung innate lymphoid cell activation and inflammation partially through CysLT1R," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32176-5
    DOI: 10.1038/s41467-022-32176-5
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    1. Kazuyo Moro & Taketo Yamada & Masanobu Tanabe & Tsutomu Takeuchi & Tomokatsu Ikawa & Hiroshi Kawamoto & Jun-ichi Furusawa & Masashi Ohtani & Hideki Fujii & Shigeo Koyasu, 2010. "Innate production of TH2 cytokines by adipose tissue-associated c-Kit+Sca-1+ lymphoid cells," Nature, Nature, vol. 463(7280), pages 540-544, January.
    2. David Artis & Hergen Spits, 2015. "The biology of innate lymphoid cells," Nature, Nature, vol. 517(7534), pages 293-301, January.
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