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Validation of Molecular Markers of Barley Net Blotch Resistance Loci on Chromosome 3H for Marker-Assisted Selection

Author

Listed:
  • Olga Afanasenko

    (All Russian Institute of Plant Protection (FSBSI VIZR), 196608 Saint Petersburg, Russia)

  • Irina Rozanova

    (N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 190000 Saint Petersburg, Russia)

  • Anastasiia Gofman

    (All Russian Institute of Plant Protection (FSBSI VIZR), 196608 Saint Petersburg, Russia)

  • Nina Lashina

    (All Russian Institute of Plant Protection (FSBSI VIZR), 196608 Saint Petersburg, Russia)

  • Fluturë Novakazi

    (Department of Plant Breeding, Swedish University of Agricultural Sciences, Sundsvägen 10, 23422 Lomma, Sweden)

  • Nina Mironenko

    (All Russian Institute of Plant Protection (FSBSI VIZR), 196608 Saint Petersburg, Russia)

  • Olga Baranova

    (All Russian Institute of Plant Protection (FSBSI VIZR), 196608 Saint Petersburg, Russia)

  • Alexandr Zubkovich

    (Republican Unitary Enterprise, The Research and Practical Center of the National Academy of Sciences of Belarus for Arable Farming, Timiriazeva Street 1, 222160 Zhodino, Belarus)

Abstract

The most widespread and harmful disease of barley is net form of net blotch caused by the ascomycete Pyrenophora teres f. teres Drechsler ( Ptt ). A cost effective and environmentally sustainable strategy for barley protection against Ptt is to develop barley cultivars possessing genetic resistance. In previous GWA analysis, we identified SNP-markers associated with a resistance locus on chromosome 3H in the interval of 45.82–54.53 cM. These SNPs have been described previously in the literature to be located within the same region of chromosome 3H. The aim of the study was to validate QTL markers controlling resistance to Ptt on chromosome 3H in this region by KASP genotyping in four F 2 populations of crosses between the resistant cultivars, Morex, Fox, and Zolo, and the accession, Local k-21578, with the susceptible barley cv. Gesine and in a doubled haploid (DH) population of Canadian Lake Shore (CLS)/Harrington. Eleven of fifteen studied markers showed high efficacy (97.5–100%) for co-segregation with resistance to Ptt in the DH population, CLS/Harrington. Three of these markers located at 54.53 cM and one at 51.27 cM were effective in two F 2 populations of crosses of Morex and Fox with susceptible cv. Gesine. These markers are also located close to each other on the physical map (442,203,921–443,119,491 bp). Apparently, in cultivars, CLS, Morex, and Fox, resistance to Ptt is determined by the same locus. Markers JHI-Hv50k-2016-166392 (47.1 cM, 112,536,071 bp), Clone ID 3255462_1 (51.63 cM, 363,531,898 bp), and Clone ID 3255462_2 (51.63 cM, 363,531,871 bp) showed high efficacy in the DH population and in the F 2 population, Local k-21578/Gesine. Apparently, at least two loci controlling Ptt resistance exist in the chromosome region of 47.0–54.3 cM: one at 46.0–48.44 cM and another at 51.27–54.8 cM. These regions were found to harbor several genes involved in important plant functions, including disease response and signaling pathways. Allele-specific PCR markers were developed based on the KASP assay data and tested on six resistant, two moderately resistant, and two susceptible barley genotypes. Four markers were found to be effective to differentiate susceptible and resistant barley genotypes. The KASP and allele-specific PCR markers associated with Ptt resistance on chromosome 3H will be useful for pyramiding resistance QTLs in barley marker-assisted selection.

Suggested Citation

  • Olga Afanasenko & Irina Rozanova & Anastasiia Gofman & Nina Lashina & Fluturë Novakazi & Nina Mironenko & Olga Baranova & Alexandr Zubkovich, 2022. "Validation of Molecular Markers of Barley Net Blotch Resistance Loci on Chromosome 3H for Marker-Assisted Selection," Agriculture, MDPI, vol. 12(4), pages 1-20, March.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:4:p:439-:d:776631
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    References listed on IDEAS

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    1. Martin Mascher & Heidrun Gundlach & Axel Himmelbach & Sebastian Beier & Sven O. Twardziok & Thomas Wicker & Volodymyr Radchuk & Christoph Dockter & Pete E. Hedley & Joanne Russell & Micha Bayer & Luke, 2017. "A chromosome conformation capture ordered sequence of the barley genome," Nature, Nature, vol. 544(7651), pages 427-433, April.
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