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Barley Stripe Mosaic Virus (BSMV) Induced MicroRNA Silencing in Common Wheat (Triticum aestivum L.)

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  • Jian Jiao
  • Yichun Wang
  • Jonathan Nimal Selvaraj
  • Fuguo Xing
  • Yang Liu

Abstract

MicroRNAs (miRNAs) play important roles in growth, development, and response to environmental changes in plants. Based on the whole-genome shotgun sequencing strategy, more and more wheat miRNAs have been annotated. Now, there is a need for an effective technology to analyse endogenous miRNAs function in wheat. We report here that the modified barley stripe mosaic virus (BSMV)-induced miRNAs silencing system can be utilized to silence miRNAs in wheat. BSMV-based miRNA silencing system is performed through BSMV-based expression of miRNA target mimics to suppress miR159a and miR3134a. The relative expression levels of mature miR159a and miR3134a decrease with increasing transcript levels of their target genes in wheat plants. In summary, the developed approach is effective in silencing endogenous miRNAs, thereby providing a powerful tool for biological function analyses of miRNA molecules in common wheat.

Suggested Citation

  • Jian Jiao & Yichun Wang & Jonathan Nimal Selvaraj & Fuguo Xing & Yang Liu, 2015. "Barley Stripe Mosaic Virus (BSMV) Induced MicroRNA Silencing in Common Wheat (Triticum aestivum L.)," PLOS ONE, Public Library of Science, vol. 10(5), pages 1-13, May.
    • Handle: RePEc:plo:pone00:0126621
    DOI: 10.1371/journal.pone.0126621
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    References listed on IDEAS

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    1. Cheng Yuan & Cui Li & Lijie Yan & Andrew O Jackson & Zhiyong Liu & Chenggui Han & Jialin Yu & Dawei Li, 2011. "A High Throughput Barley Stripe Mosaic Virus Vector for Virus Induced Gene Silencing in Monocots and Dicots," PLOS ONE, Public Library of Science, vol. 6(10), pages 1-16, October.
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