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Biogenesis of a bacterial metabolosome for propanediol utilization

Author

Listed:
  • Mengru Yang

    (University of Liverpool)

  • Nicolas Wenner

    (University of Liverpool)

  • Gregory F. Dykes

    (University of Liverpool)

  • Yan Li

    (University of Liverpool)

  • Xiaojun Zhu

    (University of Liverpool)

  • Yaqi Sun

    (University of Liverpool)

  • Fang Huang

    (University of Liverpool)

  • Jay C. D. Hinton

    (University of Liverpool)

  • Lu-Ning Liu

    (University of Liverpool
    Ocean University of China)

Abstract

Bacterial metabolosomes are a family of protein organelles in bacteria. Elucidating how thousands of proteins self-assemble to form functional metabolosomes is essential for understanding their significance in cellular metabolism and pathogenesis. Here we investigate the de novo biogenesis of propanediol-utilization (Pdu) metabolosomes and characterize the roles of the key constituents in generation and intracellular positioning of functional metabolosomes. Our results demonstrate that the Pdu metabolosome undertakes both “Shell first” and “Cargo first” assembly pathways, unlike the β-carboxysome structural analog which only involves the “Cargo first” strategy. Shell and cargo assemblies occur independently at the cell poles. The internal cargo core is formed through the ordered assembly of multiple enzyme complexes, and exhibits liquid-like properties within the metabolosome architecture. Our findings provide mechanistic insight into the molecular principles driving bacterial metabolosome assembly and expand our understanding of liquid-like organelle biogenesis.

Suggested Citation

  • Mengru Yang & Nicolas Wenner & Gregory F. Dykes & Yan Li & Xiaojun Zhu & Yaqi Sun & Fang Huang & Jay C. D. Hinton & Lu-Ning Liu, 2022. "Biogenesis of a bacterial metabolosome for propanediol utilization," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30608-w
    DOI: 10.1038/s41467-022-30608-w
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