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Decoding the stoichiometric composition and organisation of bacterial metabolosomes

Author

Listed:
  • Mengru Yang

    (University of Liverpool)

  • Deborah M. Simpson

    (University of Liverpool)

  • Nicolas Wenner

    (University of Liverpool)

  • Philip Brownridge

    (University of Liverpool)

  • Victoria M. Harman

    (University of Liverpool)

  • Jay C. D. Hinton

    (University of Liverpool)

  • Robert J. Beynon

    (University of Liverpool)

  • Lu-Ning Liu

    (University of Liverpool
    Ocean University of China)

Abstract

Some enteric bacteria including Salmonella have evolved the propanediol-utilising microcompartment (Pdu MCP), a specialised proteinaceous organelle that is essential for 1,2-propanediol degradation and enteric pathogenesis. Pdu MCPs are a family of bacterial microcompartments that are self-assembled from hundreds of proteins within the bacterial cytosol. Here, we seek a comprehensive understanding of the stoichiometric composition and organisation of Pdu MCPs. We obtain accurate stoichiometry of shell proteins and internal enzymes of the natural Pdu MCP by QconCAT-driven quantitative mass spectrometry. Genetic deletion of the major shell protein and absolute quantification reveal the stoichiometric and structural remodelling of metabolically functional Pdu MCPs. Decoding the precise protein stoichiometry allows us to develop an organisational model of the Pdu metabolosome. The structural insights into the Pdu MCP are critical for both delineating the general principles underlying bacterial organelle formation, structural robustness and function, and repurposing natural microcompartments using synthetic biology for biotechnological applications.

Suggested Citation

  • Mengru Yang & Deborah M. Simpson & Nicolas Wenner & Philip Brownridge & Victoria M. Harman & Jay C. D. Hinton & Robert J. Beynon & Lu-Ning Liu, 2020. "Decoding the stoichiometric composition and organisation of bacterial metabolosomes," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15888-4
    DOI: 10.1038/s41467-020-15888-4
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    Cited by:

    1. Mengru Yang & Nicolas Wenner & Gregory F. Dykes & Yan Li & Xiaojun Zhu & Yaqi Sun & Fang Huang & Jay C. D. Hinton & Lu-Ning Liu, 2022. "Biogenesis of a bacterial metabolosome for propanediol utilization," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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