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Encapsulation mechanisms and structural studies of GRM2 bacterial microcompartment particles

Author

Listed:
  • Gints Kalnins

    (Latvian Biomedical Research and Study Centre)

  • Eva-Emilija Cesle

    (Latvian Biomedical Research and Study Centre)

  • Juris Jansons

    (Latvian Biomedical Research and Study Centre)

  • Janis Liepins

    (University of Latvia)

  • Anatolij Filimonenko

    (Masaryk University)

  • Kaspars Tars

    (Latvian Biomedical Research and Study Centre
    University of Latvia)

Abstract

Bacterial microcompartments (BMCs) are prokaryotic organelles consisting of a protein shell and an encapsulated enzymatic core. BMCs are involved in several biochemical processes, such as choline, glycerol and ethanolamine degradation and carbon fixation. Since non-native enzymes can also be encapsulated in BMCs, an improved understanding of BMC shell assembly and encapsulation processes could be useful for synthetic biology applications. Here we report the isolation and recombinant expression of BMC structural genes from the Klebsiella pneumoniae GRM2 locus, the investigation of mechanisms behind encapsulation of the core enzymes, and the characterization of shell particles by cryo-EM. We conclude that the enzymatic core is encapsulated in a hierarchical manner and that the CutC choline lyase may play a secondary role as an adaptor protein. We also present a cryo-EM structure of a pT = 4 quasi-symmetric icosahedral shell particle at 3.3 Å resolution, and demonstrate variability among the minor shell forms.

Suggested Citation

  • Gints Kalnins & Eva-Emilija Cesle & Juris Jansons & Janis Liepins & Anatolij Filimonenko & Kaspars Tars, 2020. "Encapsulation mechanisms and structural studies of GRM2 bacterial microcompartment particles," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14205-y
    DOI: 10.1038/s41467-019-14205-y
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    Cited by:

    1. Mengru Yang & Nicolas Wenner & Gregory F. Dykes & Yan Li & Xiaojun Zhu & Yaqi Sun & Fang Huang & Jay C. D. Hinton & Lu-Ning Liu, 2022. "Biogenesis of a bacterial metabolosome for propanediol utilization," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    2. Tao Ni & Qiuyao Jiang & Pei Cing Ng & Juan Shen & Hao Dou & Yanan Zhu & Julika Radecke & Gregory F. Dykes & Fang Huang & Lu-Ning Liu & Peijun Zhang, 2023. "Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly," Nature Communications, Nature, vol. 14(1), pages 1-9, December.

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