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CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage

Author

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  • Rebecca Conners

    (University of Exeter
    University of Exeter)

  • Mathew McLaren

    (University of Exeter
    University of Exeter)

  • Urszula Łapińska

    (University of Exeter
    University of Exeter)

  • Kelly Sanders

    (University of Exeter
    University of Exeter)

  • M. Rhia L. Stone

    (The University of Queensland)

  • Mark A. T. Blaskovich

    (The University of Queensland)

  • Stefano Pagliara

    (University of Exeter
    University of Exeter)

  • Bertram Daum

    (University of Exeter
    University of Exeter)

  • Jasna Rakonjac

    (Massey University)

  • Vicki A. M. Gold

    (University of Exeter
    University of Exeter)

Abstract

The Ff family of filamentous bacteriophages infect gram-negative bacteria, but do not cause lysis of their host cell. Instead, new virions are extruded via the phage-encoded pIV protein, which has homology with bacterial secretins. Here, we determine the structure of pIV from the f1 filamentous bacteriophage at 2.7 Å resolution by cryo-electron microscopy, the first near-atomic structure of a phage secretin. Fifteen f1 pIV subunits assemble to form a gated channel in the bacterial outer membrane, with associated soluble domains projecting into the periplasm. We model channel opening and propose a mechanism for phage egress. By single-cell microfluidics experiments, we demonstrate the potential for secretins such as pIV to be used as adjuvants to increase the uptake and efficacy of antibiotics in bacteria. Finally, we compare the f1 pIV structure to its homologues to reveal similarities and differences between phage and bacterial secretins.

Suggested Citation

  • Rebecca Conners & Mathew McLaren & Urszula Łapińska & Kelly Sanders & M. Rhia L. Stone & Mark A. T. Blaskovich & Stefano Pagliara & Bertram Daum & Jasna Rakonjac & Vicki A. M. Gold, 2021. "CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26610-3
    DOI: 10.1038/s41467-021-26610-3
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    Cited by:

    1. Rebecca Conners & Rayén Ignacia León-Quezada & Mathew McLaren & Nicholas J. Bennett & Bertram Daum & Jasna Rakonjac & Vicki A. M. Gold, 2023. "Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Matteo Tassinari & Marta Rudzite & Alain Filloux & Harry H. Low, 2023. "Assembly mechanism of a Tad secretion system secretin-pilotin complex," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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