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Cryo-EM analysis of the T3S injectisome reveals the structure of the needle and open secretin

Author

Listed:
  • J. Hu

    (University of British Columbia)

  • L. J. Worrall

    (University of British Columbia
    University of British Columbia)

  • C. Hong

    (Howard Hughes Medical Institute)

  • M. Vuckovic

    (University of British Columbia)

  • C. E. Atkinson

    (University of British Columbia
    University of British Columbia)

  • N. Caveney

    (University of British Columbia)

  • Z. Yu

    (Howard Hughes Medical Institute)

  • N. C. J. Strynadka

    (University of British Columbia)

Abstract

The bacterial type III secretion system, or injectisome, is a syringe shaped nanomachine essential for the virulence of many disease causing Gram-negative bacteria. At the core of the injectisome structure is the needle complex, a continuous channel formed by the highly oligomerized inner and outer membrane hollow rings and a polymerized helical needle filament which spans through and projects into the infected host cell. Here we present the near-atomic resolution structure of a needle complex from the prototypical Salmonella Typhimurium SPI-1 type III secretion system, with local masking protocols allowing for model building and refinement of the major membrane spanning components of the needle complex base in addition to an isolated needle filament. This work provides significant insight into injectisome structure and assembly and importantly captures the molecular basis for substrate induced gating in the giant outer membrane secretin portal family.

Suggested Citation

  • J. Hu & L. J. Worrall & C. Hong & M. Vuckovic & C. E. Atkinson & N. Caveney & Z. Yu & N. C. J. Strynadka, 2018. "Cryo-EM analysis of the T3S injectisome reveals the structure of the needle and open secretin," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06298-8
    DOI: 10.1038/s41467-018-06298-8
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    Cited by:

    1. Rebecca Conners & Mathew McLaren & Urszula Łapińska & Kelly Sanders & M. Rhia L. Stone & Mark A. T. Blaskovich & Stefano Pagliara & Bertram Daum & Jasna Rakonjac & Vicki A. M. Gold, 2021. "CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Matteo Tassinari & Marta Rudzite & Alain Filloux & Harry H. Low, 2023. "Assembly mechanism of a Tad secretion system secretin-pilotin complex," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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