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Core architecture of a bacterial type II secretion system

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  • Anastasia A. Chernyatina

    (Department of Life Sciences, Imperial College)

  • Harry H. Low

    (Department of Life Sciences, Imperial College)

Abstract

Bacterial type II secretion systems (T2SSs) translocate virulence factors, toxins and enzymes across the cell outer membrane. Here we use negative stain and cryo-electron microscopy to reveal the core architecture of an assembled T2SS from the pathogen Klebsiella pneumoniae. We show that 7 proteins form a ~2.4 MDa complex that spans the cell envelope. The outer membrane complex includes the secretin PulD, with all domains modelled, and the pilotin PulS. The inner membrane assembly platform components PulC, PulE, PulL, PulM and PulN have a relative stoichiometric ratio of 2:1:1:1:1. The PulE ATPase, PulL and PulM combine to form a flexible hexameric hub. Symmetry mismatch between the outer membrane complex and assembly platform is overcome by PulC linkers spanning the periplasm, with PulC HR domains binding independently at the secretin base. Our results show that the T2SS has a highly dynamic modular architecture, with implication for pseudo-pilus assembly and substrate loading.

Suggested Citation

  • Anastasia A. Chernyatina & Harry H. Low, 2019. "Core architecture of a bacterial type II secretion system," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13301-3
    DOI: 10.1038/s41467-019-13301-3
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    Cited by:

    1. Rebecca Conners & Mathew McLaren & Urszula Łapińska & Kelly Sanders & M. Rhia L. Stone & Mark A. T. Blaskovich & Stefano Pagliara & Bertram Daum & Jasna Rakonjac & Vicki A. M. Gold, 2021. "CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Matteo Tassinari & Marta Rudzite & Alain Filloux & Harry H. Low, 2023. "Assembly mechanism of a Tad secretion system secretin-pilotin complex," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. Zhili Yu & Yaoming Wu & Muyuan Chen & Tong Huo & Wei Zheng & Steven J. Ludtke & Xiaodong Shi & Zhao Wang, 2023. "Membrane translocation process revealed by in situ structures of type II secretion system secretins," Nature Communications, Nature, vol. 14(1), pages 1-10, December.

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