IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0117676.html
   My bibliography  Save this article

A Modeling Approach to Study the Effect of Cell Polarization on Keratinocyte Migration

Author

Listed:
  • Matthias Jörg Fuhr
  • Michael Meyer
  • Eric Fehr
  • Gilles Ponzio
  • Sabine Werner
  • Hans Jürgen Herrmann

Abstract

The skin forms an efficient barrier against the environment, and rapid cutaneous wound healing after injury is therefore essential. Healing of the uppermost layer of the skin, the epidermis, involves collective migration of keratinocytes, which requires coordinated polarization of the cells. To study this process, we developed a model that allows analysis of live-cell images of migrating keratinocytes in culture based on a small number of parameters, including the radius of the cells, their mass and their polarization. This computational approach allowed the analysis of cell migration at the front of the wound and a reliable identification and quantification of the impaired polarization and migration of keratinocytes from mice lacking fibroblast growth factors 1 and 2 – an established model of impaired healing. Therefore, our modeling approach is suitable for large-scale analysis of migration phenotypes of cells with specific genetic defects or upon treatment with different pharmacological agents.

Suggested Citation

  • Matthias Jörg Fuhr & Michael Meyer & Eric Fehr & Gilles Ponzio & Sabine Werner & Hans Jürgen Herrmann, 2015. "A Modeling Approach to Study the Effect of Cell Polarization on Keratinocyte Migration," PLOS ONE, Public Library of Science, vol. 10(2), pages 1-11, February.
  • Handle: RePEc:plo:pone00:0117676
    DOI: 10.1371/journal.pone.0117676
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0117676
    Download Restriction: no

    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117676&type=printable
    Download Restriction: no

    File URL: https://libkey.io/10.1371/journal.pone.0117676?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0117676. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.