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Ghrelin Modulates Lateral Amygdala Neuronal Firing and Blocks Acquisition for Conditioned Taste Aversion

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  • Lige Song
  • Qianqian Zhu
  • Tianwei Liu
  • Ming Yu
  • Kewei Xiao
  • Qingnuan Kong
  • Renliang Zhao
  • Guo-Dong Li
  • Yu Zhou

Abstract

Ghrelin is an orexigenic brain-gut hormone promoting feeding and regulating energy metabolism in human and rodents. An increasing number of studies have reported that ghrelin and its identified receptor, the growth hormone secretagogue receptor 1a (GHS-R1a), produces remarkably wide and complex functions and biological effects on specific populations of neurons in central nervous system. In this study, we sought to explore the in vivo effects of acute ghrelin exposure on lateral amygdala (LA) neurons at the physiological and behavioral levels. In vivo extracellular single-unit recordings showed that ghrelin with the concentration of several nanomolars (nM) stimulated spontaneous firing of the LA neurons, an effect that was dose-dependent and could be blocked by co-application of a GHS-R1a antagonist D-Lys3-GHRP-6. We also found that D-Lys3-GHRP-6 inhibited spontaneous firing of the LA neurons in a dose-dependent manner, revealing that tonic GHS-R1a activity contributes to orchestrate the basal activity of the LA neurons. Behaviorally, we found that microinfusion of ghrelin (12 ng) into LA before training interfered with the acquisition of conditioned taste aversion (CTA) as tested at 24 h after conditioning. Pre-treatment with either purified IgG against GHS-R1a or GHS-R1a antagonist blocked ghrelin’s effect on CTA memory acquisition. Ghrelin (12 ng) had no effect on CTA memory consolidation or the expression of acquired CTA memory; neither did it affect the total liquid consumption of tested rats. Altogether, our data indicated that ghrelin locally infused into LA blocks acquisition of CTA and its modulation effects on neuronal firing may be involved in this process.

Suggested Citation

  • Lige Song & Qianqian Zhu & Tianwei Liu & Ming Yu & Kewei Xiao & Qingnuan Kong & Renliang Zhao & Guo-Dong Li & Yu Zhou, 2013. "Ghrelin Modulates Lateral Amygdala Neuronal Firing and Blocks Acquisition for Conditioned Taste Aversion," PLOS ONE, Public Library of Science, vol. 8(6), pages 1-12, June.
    • Handle: RePEc:plo:pone00:0065422
    DOI: 10.1371/journal.pone.0065422
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    2. Matthias Tschöp & David L. Smiley & Mark L. Heiman, 2000. "Ghrelin induces adiposity in rodents," Nature, Nature, vol. 407(6806), pages 908-913, October.
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