IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0177995.html
   My bibliography  Save this article

Ghrelin modulates testicular damage in a cryptorchid mouse model

Author

Listed:
  • Enrica Bianchi
  • Kim Boekelheide
  • Mark Sigman
  • Susan J Hall
  • Kathleen Hwang

Abstract

Cryptorchidism or undescended testis (UDT) is a common congenital abnormality associated with increased risk for developing male infertility and testicular cancer. This study elucidated the effects of endogenous ghrelin or growth hormone secretagogue receptor (GHSR) deletion on mouse reproductive performance and evaluated the ability of ghrelin to prevent testicular damage in a surgical cryptorchid mouse model. Reciprocal matings with heterozygous/homozygous ghrelin and GHSR knockout mice were performed. Litter size and germ cell apoptosis were recorded and testicular histological evaluations were performed. Wild type and GHSR knockout adult mice were subjected to creation of unilateral surgical cryptorchidism that is a model of heat-induced germ cell death. All mice were randomly separated into two groups: treatment with ghrelin or with saline. To assess testicular damage, the following endpoints were evaluated: testis weight, seminiferous tubule diameter, percentage of seminiferous tubules with spermatids and with multinucleated giant cells. Our findings indicated that endogenous ghrelin deletion altered male fertility. Moreover, ghrelin treatment ameliorated the testicular weight changes caused by surgically induced cryptorchidism. Testicular histopathology revealed a significant preservation of spermatogenesis and seminiferous tubule diameter in the ghrelin-treated cryptorchid testes of GHSR KO mice, suggesting that this protective effect of ghrelin was mediated by an unknown mechanism. In conclusion, ghrelin therapy could be useful to suppress testicular damage induced by hyperthermia, and future investigations will focus on the underlying mechanisms by which ghrelin mitigates testicular damage.

Suggested Citation

  • Enrica Bianchi & Kim Boekelheide & Mark Sigman & Susan J Hall & Kathleen Hwang, 2017. "Ghrelin modulates testicular damage in a cryptorchid mouse model," PLOS ONE, Public Library of Science, vol. 12(5), pages 1-17, May.
    • Handle: RePEc:plo:pone00:0177995
    DOI: 10.1371/journal.pone.0177995
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177995
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0177995&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0177995?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Masayasu Kojima & Hiroshi Hosoda & Yukari Date & Masamitsu Nakazato & Hisayuki Matsuo & Kenji Kangawa, 1999. "Ghrelin is a growth-hormone-releasing acylated peptide from stomach," Nature, Nature, vol. 402(6762), pages 656-660, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. K. Tanaka & T. Takizawa & O. Oki & K. Fukawa & T. Ito & M. Miyabe & H. Mannen & Y. Kurosawa & K. Hirose, 2015. "Allele frequency for c.335 A>C polymorphisms in porcine ghrelin/obestatin prepropeptide gene and association analysis with performance traits in various pig breeds," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 60(9), pages 411-416.
    2. Qian Wang, 2017. "The Ghrelin/GHS-R1a Pathway is Involved in the Mechanism of the CTA-regulated Neuronal Loop," International Journal of Sciences, Office ijSciences, vol. 6(01), pages 64-72, January.
    3. Michelle L Johnson & M Jill Saffrey & Victoria J Taylor, 2016. "Plasma Ghrelin Concentrations Were Altered with Oestrous Cycle Stage and Increasing Age in Reproductively Competent Wistar Females," PLOS ONE, Public Library of Science, vol. 11(11), pages 1-12, November.
    4. A. Lotfi & H. Aghdam-Shahryar & J. Ghiasi-Ghalehkandi & H. Kaiya & N. Maheri-Sis, 2011. "Effect of in ovo ghrelin administration on subsequent serum insulin and glucose levels in newly-hatched chicks," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 56(8), pages 377-380.
    5. Mohammed Saeed Al-Ayed & Khaled Sadeq Al-Shaibari & Dhafer Alshehri & Mohammed Jamaan Alzahrani & Iman Nasser & Hamdan Saad Alaamri & Wed Ahmad Alaseeri & Ahmed A. Mahfouz & Saeed Ali Alsareii & Ahmed, 2020. "Serum Ghrelin Levels in Saudi Obese Asthmatic School-Children—Correlation with Interleukin-4, Interleukin-5, and Interleukin-21," IJERPH, MDPI, vol. 17(5), pages 1-11, March.
    6. Gerrit Vortmeier & Stephanie H DeLuca & Sylvia Els-Heindl & Constance Chollet & Holger A Scheidt & Annette G Beck-Sickinger & Jens Meiler & Daniel Huster, 2015. "Integrating Solid-State NMR and Computational Modeling to Investigate the Structure and Dynamics of Membrane-Associated Ghrelin," PLOS ONE, Public Library of Science, vol. 10(3), pages 1-28, March.
    7. Nazan Yurtcu & Canan Soyer Caliskan & Huri Guvey & Samettin Celik & Safak Hatirnaz & Andrea Tinelli, 2022. "Predictive and Diagnostic Value of Serum Adipokines in Pregnant Women with Intrahepatic Cholestasis," IJERPH, MDPI, vol. 19(4), pages 1-13, February.
    8. Mahalaqua Nazli Khatib & Anuraj Shankar & Richard Kirubakaran & Kingsley Agho & Padam Simkhada & Shilpa Gaidhane & Deepak Saxena & Unnikrishnan B & Dilip Gode & Abhay Gaidhane & Syed Quazi Zahiruddin, 2015. "Effect of Ghrelin on Mortality and Cardiovascular Outcomes in Experimental Rat and Mice Models of Heart Failure: A Systematic Review and Meta-Analysis," PLOS ONE, Public Library of Science, vol. 10(5), pages 1-21, May.
    9. Amany M Basuny & Moustafa A AboelAnin & Eman A Hamed, 2020. "Structure and Physiological Functions of Ghrelin," Biomedical Journal of Scientific & Technical Research, Biomedical Research Network+, LLC, vol. 31(2), pages 24085-24092, October.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0177995. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.